• Immunofluorescence analysis of HeLa cells using P2RX4 Rabbit polyclonal antibody (STJ11105551) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using P2RX4 Rabbit polyclonal antibody (STJ11105551) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded Rat small intestine using P2RX4 Rabbit polyclonal antibody (STJ11105551) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of U-2 OS cells using P2RX4 Rabbit polyclonal antibody (STJ11105551) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-P2RX4 antibody (111-211) (STJ11105551)

SKU:
STJ11105551

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-P2x Purinoceptor 4 (111-211) is suitable for use in Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: P2RX4
Gene ID: 5025
Uniprot ID: P2RX4_HUMAN
Immunogen Region: 111-211
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 111-211 of human P2RX4 (NP_002551.2).
Immunogen Sequence: QTQGLCPEIPDATTVCKSDA SCTAGSAGTHSNGVSTGRCV AFNGSVKTCEVAAWCPVEDD THVPQPAFLKAAENFTLLVK NNIWYPKFNFSKRNILPNIT T
Function ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and calcium. Activated by extracellularly released ATP, it plays multiple role in immunity and central nervous system physiology. Plays a key role in initial steps of T-cell activation and Ca(2+) microdomain formation. Participates also in basal T-cell activity without TCR/CD3 stimulation. Promotes the differentiation and activation of Th17 cells via expression of retinoic acid-related orphan receptor C/RORC. Upon activation, drives microglia motility via the PI3K/Akt pathway. Could also function as an ATP-gated cation channel of lysosomal membranes.
Protein Name P2x Purinoceptor 4
P2x4
Atp Receptor
Purinergic Receptor
Database Links Reactome: R-HSA-139853
Reactome: R-HSA-418346
Reactome: R-HSA-9660826
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Lysosome Membrane
Alternative Antibody Names Anti-P2x Purinoceptor 4 antibody
Anti-P2x4 antibody
Anti-Atp Receptor antibody
Anti-Purinergic Receptor antibody
Anti-P2RX4 antibody

Information sourced from Uniprot.org

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