• Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L. ) variety Zhonghua 11, using OsRGB1 Rabbit polyclonal antibody (STJ11103850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-OsRGB1 antibody (1-300 aa) (STJ11103850)

SKU:
STJ11103850

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Host: Rabbit
Applications: WB/ELISA
Reactivity: O.sativa
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-300 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of Oryza sativa OsRGB1 (Q40687).
Immunogen Sequence: MASVAELKEKHAAATASVNS LRERLRQRRQMLLDTDVERY SRTQGRTPVSFNPTDLVCCR TLQGHSGKVYSLDWTPEKNW IVSASQDGRLIVWNALTSQK THAIKLHCPWVMTCAFAPNG QSVACGGLDSACSIFNLNSQ ADRDGNIPVSRILTGHKGYV SSCQYVPDQETRLITSSGDQ TCVLWDVTTGQRISIFGGEF PSGHTADVLSLSINSSNSNM FVSGSCDATVRLWDIRIAS
Background Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Information sourced from Uniprot.org

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