| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | OSBPL3 |
| Gene ID: | 26031 |
| Uniprot ID: | OSBL3_HUMAN |
| Immunogen Region: | 370-520 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 370-520 of human OSBPL3 (NP_056365.1). |
| Immunogen Sequence: | SSSPSAQVIGLKNALSSALA QNTDLKERLRRIHAESLLLD SPAVAKSGDNLAEENSRDEN RALVHQLSNESRLSITDSLS EFFDAQEVLLSPSSSENEIS DDDSYVSDISDNLSLDNLSN DLDNERQTLGPVLDSGREAK SRRRTCLPAPC |
| Tissue Specificity | Expressed in a subset of small lymphocytes (at protein level). Expressed at high concentration in kidney, lymph node and thymus. Expressed at moderate concentration in stomach, jejunum, ileum, appendix, spleen, leukocytes, trachea, lung and thyroid gland. Expressed at low concentration in whole brain, esophagus, duodenum, ileocecum, colon, skeletal muscle, bone marrow, placenta and mammary gland. Isoform 1a, isoform 1b, isoform 1c and isoform 1d are highly expressed in brain, bone marrow, colon, kidney, lung, skeletal muscle, spleen, thymus and thyroid. Not expressed in heart and liver. Isoform 2a, isoform 2b, isoform 2c and isoform 2d are expressed in brain, bone marrow, kidney, skeletal muscle, spleen, thymus and thyroid. Not expressed in heart, liver and lung. |
| Post Translational Modifications | Phosphorylation is enhanced in vitro by phorbol-12-myristate-13-acetate (PMA), forskolin and calcium ionophore A23187. Phosphorylation seems to be stimulated in conditions of low cell-cell (or cell-matrix) adhesion. |
| Function | Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes. Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments. The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With VAPA, may regulate ER morphology. Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion. Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3. Also binds 25-hydroxycholesterol and cholesterol. |
| Protein Name | Oxysterol-Binding Protein-Related Protein 3Orp-3Osbp-Related Protein 3 |
| Database Links | Reactome: R-HSA-192105 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmCytosolCell MembraneCell ProjectionFilopodium TipNucleus MembraneCo-Localizes With Osbpl6 At Contact Sites Between The Plasma Membrane And The Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Oxysterol-Binding Protein-Related Protein 3 antibodyAnti-Orp-3 antibodyAnti-Osbp-Related Protein 3 antibodyAnti-OSBPL3 antibodyAnti-KIAA0704 antibodyAnti-ORP3 antibodyAnti-OSBP3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
