| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | OSBP |
| Gene ID: | 5007 |
| Uniprot ID: | OSBP1_HUMAN |
| Immunogen Region: | 508-807 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 508-807 of human OSBP (NP_002547.1). |
| Immunogen Sequence: | LEENGYRSLCEQVSHHPPAA AHHAESKNGWTLRQEIKITS KFRGKYLSIMPLGTIHCIFH ATGHHYTWKKVTTTVHNIIV GKLWIDQSGEIDIVNHKTGD KCNLKFVPYSYFSRDVARKV TGEVTDPSGKVHFALLGTWD EKMECFKVQPVIGENGGDAR QRGHEAEESRVMLWKRNPLP KNAENMYYFSELALTLNAWE SGTAPTDSRLRPDQRLMENG RWDEANAEKQRLEEKQRLS |
| Tissue Specificity | Widely expressed. |
| Function | Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability. |
| Protein Name | Oxysterol-Binding Protein 1 |
| Database Links | Reactome: R-HSA-1660661Reactome: R-HSA-192105 |
| Cellular Localisation | CytoplasmCytosolPerinuclear RegionGolgi Apparatus MembranePeripheral Membrane ProteinEndoplasmic Reticulum MembraneGolgi ApparatusTrans-Golgi NetworkPredominantly Cytosolic |
| Alternative Antibody Names | Anti-Oxysterol-Binding Protein 1 antibodyAnti-OSBP antibodyAnti-OSBP1 antibody |
Information sourced from Uniprot.org