| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Orai1 (C-Term) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
ORAI1 |
| Gene ID: |
84876 |
| Uniprot ID: |
ORAI1_HUMAN |
| Immunogen Region: |
C-Term |
| Specificity: |
Specific for Orai1. |
| Immunogen: |
A synthetic peptide from the c-terminal region of rat Orai1 (TMEM142a) conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in mouse and human. |
| Post Translational Modifications | N-glycosylated. N-glycosylation inhibits channel activity in T cells. Ubiquitinated. Cys-195 is oxidated, leading to inactivation of channel activity. |
| Function | Pore-forming subunit of two major inward rectifying Ca(2+) channels at the plasma membrane: Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels (Probable). Assembles with ORAI2 and ORAI3 to form hexameric CRAC channels that mediate Ca(2+) influx upon depletion of endoplasmic reticulum Ca(2+) store and channel activation by Ca(2+) sensor STIM1, a process known as store-operated Ca(2+) entry (SOCE). Various pore subunit combinations may account for distinct CRAC channel spatiotemporal and cell-type specific dynamics. ORAI1 mainly contributes to the generation of Ca(2+) plateaus involved in sustained Ca(2+) entry and is dispensable for cytosolic Ca(2+) oscillations, whereas ORAI2 and ORAI3 generate oscillatory patterns. CRAC channels assemble in Ca(2+) signaling microdomains where Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT transcription factors recruited to ORAI1 via AKAP5. Activates NFATC2/NFAT1 and NFATC3/NFAT4-mediated transcriptional responses. CRAC channels are the main pathway for Ca(2+) influx in T cells and promote the immune response to pathogens by activating NFAT-dependent cytokine and chemokine transcription. Assembles with ORAI3 to form channels that mediate store-independent Ca(2+) influx in response to inflammatory metabolites arachidonate or its derivative leukotriene C4, termed ARC and LRC channels respectively. Plays a prominent role in Ca(2+) influx at the basolateral membrane of mammary epithelial cells independently of the Ca(2+) content of endoplasmic reticulum or Golgi stores. May mediate transepithelial transport of large quantities of Ca(2+) for milk secretion. Isoform alpha: Pore-forming subunit of both CRAC and ARC channels. Couples Ca(2+) influx to NFAT-mediated transcriptional responses. Isoform beta: Pore-forming subunit of CRAC channels exclusively. |
| Protein Name | Calcium Release-Activated Calcium Channel Protein 1Protein Orai-1Transmembrane Protein 142a |
| Database Links | Reactome: R-HSA-139853Reactome: R-HSA-5578775Reactome: R-HSA-983695 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneUpon Store DepletionColocalizes With Stim1 In Membrane Punctae At Er-Pm JunctionsIsoform Alpha: Cell MembraneIsoform Beta: Cell Membrane |
| Alternative Antibody Names | Anti-Calcium Release-Activated Calcium Channel Protein 1 antibodyAnti-Protein Orai-1 antibodyAnti-Transmembrane Protein 142a antibodyAnti-ORAI1 antibodyAnti-CRACM1 antibodyAnti-TMEM142A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
