| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-OPN5 (200-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
OPN5 |
| Gene ID: |
221391 |
| Uniprot ID: |
OPN5_HUMAN |
| Immunogen Region: |
200-300 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human OPN5 (NP_859528.1). |
| Immunogen Sequence: |
ILNILFFCLLLPTAVIVFSY VKIIAKVKSSSKEVAHFDSR IHSSHVLEMKLTKVAMLICA GFLIAWIPYAVVSVWSAFGR PDSIPIQLSVVPTLLAKSAA M |
| Tissue Specificity | Detected in brain and retina and cell lines derived from neural retina. |
| Post Translational Modifications | It is uncertain whether Cys-315 or Cys-316 is palmitoylated. |
| Function | G-protein coupled receptor which selectively activates G(i) type G proteins via ultraviolet A (UVA) light-mediated activation in the retina. Preferentially binds the chromophore 11-cis retinal and is a bistable protein that displays emission peaks at 380 nm (UVA light) and 470 nm (blue light). Required for the light-response in the inner plexiform layer, and contributes to the regulation of the light-response in the nerve fiber layer, via phosphorylated DAT/SLC6A3 dopamine uptake. Involved in local corneal and retinal circadian rhythm photoentrainment via modulation of the UVA light-induced phase-shift of the retina clock. Acts as a circadian photoreceptor in the outer ear, via modulation of circadian clock-gene expression in response to violet light during the light-to-dark transition phase and night phase of the circadian cycle. Required in the retina to negatively regulate hyaloid vessel regression during postnatal development via light-dependent OPN5-SLC32A1-DRD2-VEGFR2 signaling. Involved in the light-dependent regulation of retina and vitreous compartment dopamine levels. |
| Protein Name | Opsin-5G-Protein Coupled Receptor 136G-Protein Coupled Receptor Pgr12NeuropsinTransmembrane Protein 13 |
| Database Links | Reactome: R-HSA-418594Reactome: R-HSA-419771 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Opsin-5 antibodyAnti-G-Protein Coupled Receptor 136 antibodyAnti-G-Protein Coupled Receptor Pgr12 antibodyAnti-Neuropsin antibodyAnti-Transmembrane Protein 13 antibodyAnti-OPN5 antibodyAnti-GPR136 antibodyAnti-PGR12 antibodyAnti-TMEM13 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of U-2 OS cells using [KO Validated] ERK2 Rabbit polyclonal antibody (STJ113009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/92725/373210/STJ113009_1__36729.1713139394.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] ERK2 Rabbit polyclonal antibody (STJ113009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
