• Western blot analysis of various lysates, using OMA1 Rabbit polyclonal antibody (STJ11105230) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-OMA1 antibody (421-520) (STJ11105230)

SKU:
STJ11105230

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Metalloendopeptidase Oma1-Mitochondrial (421-520) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OMA1
Gene ID: 115209
Uniprot ID: OMA1_HUMAN
Immunogen Region: 421-520
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 421-520 of human OMA1 (NP_660286.1).
Immunogen Sequence: EFVDSLHGQPKMPEWLSTHP SHGNRVEYLDRLIPQALKIR EMCNCPPLSNPDPRLLFKLS TKHFLEESEKEDLNITKKQK MDTLPIQKQEQIPLTYIVEK
Tissue Specificity Widely expressed, with strong expression in the heart, skeletal muscle, kidney and liver.
Post Translational Modifications May form a redox-dependent disulfide bond. Exists in a semi-oxidized state and is activated by prolonged hypoxia. Autocatalytically cleaved in response to mitochondrial depolarization both at the N-terminus and C-terminus to generate the short active form (S-OMA1). Autocatalytic processing at the C-terminus takes place at residues 447-456. The S-OMA1 form is unstable. Degradaded by YMEL1 in response to membrane depolarization. Protein turnover is regulated by prohibitin (PHB and PHB2), which promotes degradation of OMA1 in a cardiolipin-binding manner.
Function Metalloprotease that is part of the quality control system in the inner membrane of mitochondria. Activated in response to various mitochondrial stress, leading to the proteolytic cleavage of target proteins, such as OPA1, UQCC3 and DELE1. Following stress conditions that induce loss of mitochondrial membrane potential, mediates cleavage of OPA1 at S1 position, leading to OPA1 inactivation and negative regulation of mitochondrial fusion. Also acts as a regulator of apoptosis: upon BAK and BAX aggregation, mediates cleavage of OPA1, leading to the remodeling of mitochondrial cristae and allowing the release of cytochrome c from mitochondrial cristae. In depolarized mitochondria, may also act as a backup protease for PINK1 by mediating PINK1 cleavage and promoting its subsequent degradation by the proteasome. May also cleave UQCC3 in response to mitochondrial depolarization. Also acts as an activator of the integrated stress response (ISR): in response to mitochondrial stress, mediates cleavage of DELE1 to generate the processed form of DELE1 (S-DELE1), which translocates to the cytosol and activates EIF2AK1/HRI to trigger the ISR. Its role in mitochondrial quality control is essential for regulating lipid metabolism as well as to maintain body temperature and energy expenditure under cold-stress conditions. Binds cardiolipin, possibly regulating its protein turnover. Required for the stability of the respiratory supercomplexes.
Protein Name Metalloendopeptidase Oma1 - Mitochondrial
Metalloprotease-Related Protein 1
Mprp-1
Overlapping With The M-Aaa Protease 1 Homolog
Database Links Reactome: R-HSA-169911
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Metalloendopeptidase Oma1 - Mitochondrial antibody
Anti-Metalloprotease-Related Protein 1 antibody
Anti-Mprp-1 antibody
Anti-Overlapping With The M-Aaa Protease 1 Homolog antibody
Anti-OMA1 antibody
Anti-MPRP1 antibody

Information sourced from Uniprot.org

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