• Immunofluorescence analysis of PC-12 cells using OLA1 Rabbit polyclonal antibody (STJ113271) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using OLA1 Rabbit polyclonal antibody (STJ113271) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of OLA1 in paraffin-embedded human lung cancer using OLA1 Rabbit polyclonal antibody (STJ113271) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of OLA1 in paraffin-embedded human breast cancer using OLA1 Rabbit polyclonal antibody (STJ113271) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using OLA1 Rabbit polyclonal antibody (STJ113271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-OLA1 antibody (130-310) (STJ113271)

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STJ113271

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OLA1 (130-310) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OLA1
Gene ID: 29789
Uniprot ID: OLA1_HUMAN
Immunogen Region: 130-310
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-310 of human OLA1 (NP_037473.3).
Immunogen Sequence: DDITHVEGSVDPIRDIEIIH EELQLKDEEMIGPIIDKLEK VAVRGGDKKLKPEYDIMCKV KSWVIDQKKPVRFYHDWNDK EIEVLNKHLFLTSKPMVYLV NLSEKDYIRKKNKWLIKIKE WVDKYDPGALVIPFSGALEL KLQELSAEERQKYLEANMTQ SALPKIIKAGFAALQLEYFF T
Tissue Specificity Expressed in all tissues tested but its expression is more abundant in testis, liver, lung, and brain. Overexpressed in several malignancies, including cancers of the colon, rectum, ovary, lung, stomach, and uterus.
Function Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.
Protein Name Obg-Like Atpase 1
Dna Damage-Regulated Overexpressed In Cancer 45
Doc45
Gtp-Binding Protein 9
Database Links Reactome: R-HSA-114608
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Predominantly Cytoplasmic
Shuttles Between The Nucleus And The Cytoplasm
Alternative Antibody Names Anti-Obg-Like Atpase 1 antibody
Anti-Dna Damage-Regulated Overexpressed In Cancer 45 antibody
Anti-Doc45 antibody
Anti-Gtp-Binding Protein 9 antibody
Anti-OLA1 antibody
Anti-GTPBP9 antibody
Anti-PRO2455 antibody
Anti-PTD004 antibody

Information sourced from Uniprot.org

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