| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:5000IHC-P:1:100-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | OGN |
| Gene ID: | 4969 |
| Uniprot ID: | MIME_HUMAN |
| Immunogen Region: | 20-298 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-298 of human Osteoglycin (OGN) (NP_054776.1). |
| Immunogen Sequence: | APPTQQDSRIIYDYGTDNFE ESIFSQDYEDKYLDGKNIKE KETVIIPNEKSLQLQKDEAI TPLPPKKENDEMPTCLLCVC LSGSVYCEEVDIDAVPPLPK ESAYLYARFNKIKKLTAKDF ADIPNLRRLDFTGNLIEDIE DGTFSKLSLLEELSLAENQL LKLPVLPPKLTLFNAKYNKI KSRGIKANAFKKLNNLTFLY LDHNALESVPLNLPESLRVI HLQFNNIASITDDTFCKAN |
| Tissue Specificity | Bone. |
| Post Translational Modifications | O-glycosylated with a core 1 or possibly core 8 glycan. Contains keratan sulfate. |
| Function | Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2. |
| Protein Name | MimecanOsteoglycinOsteoinductive FactorOif |
| Database Links | Reactome: R-HSA-2022854Reactome: R-HSA-2022857Reactome: R-HSA-3656225Reactome: R-HSA-3656243Reactome: R-HSA-3656244 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Names | Anti-Mimecan antibodyAnti-Osteoglycin antibodyAnti-Osteoinductive Factor antibodyAnti-Oif antibodyAnti-OGN antibodyAnti-OIF antibodyAnti-SLRR3A antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and CDK2 knockout (KO) 293T cells, using [KO Validated] CDK2 Rabbit pAb (STJ119973) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98758/410534/STJ119973_1__53913.1744897009.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CDK2 knockout (KO) 293T cells, using [KO Validated] CDK2 Rabbit pAb (STJ119973) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.")
