• Immunofluorescence analysis of U-2 OS cells using Osteoglycin (OGN) antibody (STJ28762) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using Osteoglycin (OGN) antibody (STJ28762) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Osteoglycin (OGN) antibody (STJ28762) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using Osteoglycin (OGN) Rabbit polyclonal antibody (STJ28762) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-OGN antibody (20-298) (STJ28762)

SKU:
STJ28762

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OGN (20-298) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OGN
Gene ID: 4969
Uniprot ID: MIME_HUMAN
Immunogen Region: 20-298
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-298 of human Osteoglycin (OGN) (NP_054776.1).
Immunogen Sequence: APPTQQDSRIIYDYGTDNFE ESIFSQDYEDKYLDGKNIKE KETVIIPNEKSLQLQKDEAI TPLPPKKENDEMPTCLLCVC LSGSVYCEEVDIDAVPPLPK ESAYLYARFNKIKKLTAKDF ADIPNLRRLDFTGNLIEDIE DGTFSKLSLLEELSLAENQL LKLPVLPPKLTLFNAKYNKI KSRGIKANAFKKLNNLTFLY LDHNALESVPLNLPESLRVI HLQFNNIASITDDTFCKAN
Tissue Specificity Bone.
Post Translational Modifications O-glycosylated with a core 1 or possibly core 8 glycan. Contains keratan sulfate.
Function Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.
Protein Name Mimecan
Osteoglycin
Osteoinductive Factor
Oif
Database Links Reactome: R-HSA-2022854
Reactome: R-HSA-2022857
Reactome: R-HSA-3656225
Reactome: R-HSA-3656243
Reactome: R-HSA-3656244
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Mimecan antibody
Anti-Osteoglycin antibody
Anti-Osteoinductive Factor antibody
Anti-Oif antibody
Anti-OGN antibody
Anti-OIF antibody
Anti-SLRR3A antibody

Information sourced from Uniprot.org

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