• STJ70849 (2µg/ml) staining of HEK293 (A) , and A431 (B) nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70849 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70849 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70849 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-OGFR antibody (C-Term) (STJ70849)

SKU:
STJ70849-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-OGFR (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:4000.
WB: Approx 75kDa band observed in nuclear cell lysates of HEK293 and A431 (calculated MW of 73.3kDa according to NP_031372.2) Recommended concentration: 1-3µg/ml. Primary incubation 1 hour
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: OGFR
Gene ID: 11054
Uniprot ID: OGFR_HUMAN
Immunogen Region: C-Term
Accession Number: NP_031372.2
Immunogen Sequence: QDAEVESSAKSGK
Function Receptor for opioid growth factor (OGF), also known as Met-enkephalin. Seems to be involved in growth regulation.
Protein Name Opioid Growth Factor Receptor
Ogfr
Protein 7-60
Zeta-Type Opioid Receptor
Cellular Localisation Cytoplasm
Nucleus
The Ogf/Ogfr Complex Is Probably Translocated To The Nucleus
Alternative Antibody Names Anti-Opioid Growth Factor Receptor antibody
Anti-Ogfr antibody
Anti-Protein 7-60 antibody
Anti-Zeta-Type Opioid Receptor antibody
Anti-OGFR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance