• Western blot analysis of Mouse lung, using Occludin Rabbit polyclonal antibody (STJ111161) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-OCLN antibody (400-522) (STJ111161)

SKU:
STJ111161

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Occludin (400-522) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OCLN
Gene ID: 100506658
Uniprot ID: OCLN_HUMAN
Immunogen Region: 400-522
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-522 of human Occludin (NP_002529.1).
Immunogen Sequence: TDYTTGGESCDELEEDWIRE YPPITSDQQRQLYKRNFDTG LQEYKSLQSELDEINKELSR LDKELDDYREESEEYMAAAD EYNRLKQVKGSADYKSKKNH CKQLKSKLSHIKKMVGDYDR QKT
Tissue Specificity Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Post Translational Modifications Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1. (Microbial infection) Cleaved by S.pyogenes SpeB protease.leading to its degradation. Degradation by SpeB promotes bacterial translocation across the host epithelial barrier.
Function May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.
Protein Name Occludin
Database Links Reactome: R-HSA-351906
Reactome: R-HSA-8935964
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Junction
Tight Junction
Alternative Antibody Names Anti-Occludin antibody
Anti-OCLN antibody

Information sourced from Uniprot.org

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