• Western blot analysis of extracts of various cell lines, using OAS3 antibody (STJ113646) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of U-2 OS cells using OAS3 Rabbit polyclonal antibody (STJ113646) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using OAS3 antibody (STJ113646) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using OAS3 Rabbit polyclonal antibody (STJ113646) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using OAS3 antibody (STJ113646) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using OAS3 Rabbit polyclonal antibody (STJ113646) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-OAS3 antibody (320-620) (STJ113646)

SKU:
STJ113646

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OAS3 (320-620) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OAS3
Gene ID: 4940
Uniprot ID: OAS3_HUMAN
Immunogen Region: 320-620
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 320-620 of human OAS3 (NP_006178.2).
Immunogen Sequence: AASCYDHPCFLRGMGDPVQS WKGPGLPRAGCSGLGHPIQL DPNQKTPENSKSLNAVYPRA GSKPPSCPAPGPTGAASIVP SVPGMALDLSQIPTKELDRF IQDHLKPSPQFQEQVKKAID IILRCLHENCVHKASRVSKG GSFGRGTDLRDGCDVELIIF LNCFTDYKDQGPRRAEILDE MRAQLESWWQDQVPSLSLQF PEQNVPEALQFQLVSTALKS WTDVSLLPAFDAVGQLSSG
Tissue Specificity Present at high level in placenta trophoblast.
Function Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes preferentially dimers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. Displays antiviral activity against Chikungunya virus (CHIKV), Dengue virus, Sindbis virus (SINV) and Semliki forest virus (SFV).
Protein Name 2'-5'-Oligoadenylate Synthase 3
(2-5'Oligo(A Synthase 3
2-5a Synthase 3
P100 Oas
P100oas
Database Links Reactome: R-HSA-877300
Reactome: R-HSA-8983711
Reactome: R-HSA-909733
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-2'-5'-Oligoadenylate Synthase 3 antibody
Anti-(2-5'Oligo(A Synthase 3 antibody
Anti-2-5a Synthase 3 antibody
Anti-P100 Oas antibody
Anti-P100oas antibody
Anti-OAS3 antibody
Anti-P antibody
Anti-OKcl.4 antibody

Information sourced from Uniprot.org

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