• Western blot analysis of various lysates using NUP54 Rabbit polyclonal antibody (STJ117649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NUP54 antibody (100-400) (STJ117649)

SKU:
STJ117649

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUP54 (100-400) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUP54
Gene ID: 53371
Uniprot ID: NUP54_HUMAN
Immunogen Region: 100-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human NUP54 (NP_059122.2).
Immunogen Sequence: LGGLFSQPTQAPTQSNQLIN TASALSAPTLLGDERDAILA KWNQLQAFWGTGKGYFNNNI PPVEFTQENPFCRFKAVGYS CMPSNKDEDGLVVLVFNKKE TEIRSQQQQLVESLHKVLGG NQTLTVNVEGTKTLPDDQTE VVIYVVERSPNGTSRRVPAT TLYAHFEQANIKTQLQQLGV TLSMTRTELSPAQIKQLLQN PPAGVDPIIWEQAKVDNPDS EKLIPVPMVGFKELLRRLK
Post Translational Modifications O-glycosylated.
Function Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane.
Protein Name Nucleoporin P54
54 Kda Nucleoporin
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-159227
Reactome: R-HSA-159230
Reactome: R-HSA-159231
Reactome: R-HSA-159236
Reactome: R-HSA-165054
Reactome: R-HSA-168271
Reactome: R-HSA-168276
Reactome: R-HSA-168325
Reactome: R-HSA-168333
Reactome: R-HSA-170822
Reactome: R-HSA-180746
Reactome: R-HSA-180910
Reactome: R-HSA-191859
Reactome: R-HSA-3108214
Reactome: R-HSA-3232142
Reactome: R-HSA-3301854
Reactome: R-HSA-3371453
Reactome: R-HSA-4085377
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4615885
Reactome: R-HSA-5578749
Reactome: R-HSA-5619107
Reactome: R-HSA-6784531
Reactome: R-HSA-9609690
Reactome: R-HSA-9610379
Reactome: R-HSA-9615933
Reactome: R-HSA-9705671
Cellular Localisation Nucleus
Nuclear Pore Complex
Nucleus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Nucleoplasmic Side
Biased Towards Cytoplasmic Side
Central Region Of The Nuclear Pore Complex
Within The Transporter
Alternative Antibody Names Anti-Nucleoporin P54 antibody
Anti-54 Kda Nucleoporin antibody
Anti-NUP54 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance