• Western blot analysis of extracts of various cell lines, using NUMB antibody (STJ111671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of PC-12 cells using NUMB Rabbit polyclonal antibody (STJ111671) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using NUMB antibody (STJ111671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of NIH/3T3 cells using NUMB Rabbit polyclonal antibody (STJ111671) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human liver using NUMB rabbit polyclonal antibody (STJ111671) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using NUMB Rabbit polyclonal antibody (STJ111671) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human lung cancer using NUMB rabbit polyclonal antibody (STJ111671) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using NUMB Rabbit polyclonal antibody (STJ111671) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using NUMB rabbit polyclonal antibody (STJ111671) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NUMB antibody (STJ111671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded rat kidney using NUMB rabbit polyclonal antibody (STJ111671) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NUMB antibody (STJ111671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of L929 cells using NUMB antibody (STJ111671) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NUMB antibody (521-640) (STJ111671)

SKU:
STJ111671

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUMB (521-640) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUMB
Gene ID: 8650
Uniprot ID: NUMB_HUMAN
Immunogen Region: 521-640
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 521-640 of human NUMB (NP_003735.3).
Immunogen Sequence: SQMVANVFGTAGHPQAAHPH QSPSLVRQQTFPHYEASSAT TSPFFKPPAQHLNGSAAFNG VDDGRLASADRHTEVPTGTC PVDPFEAQWAALENKSKQRT NPSPTNPFSSDLQKTFEIEL
Post Translational Modifications Phosphorylated on Ser-276 and Ser-295 by CaMK1. Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation. Ubiquitinated.mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Function Regulates clathrin-mediated receptor endocytosis. Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
Protein Name Protein Numb Homolog
H-Numb
Protein S171
Database Links Reactome: R-HSA-2122948
Reactome: R-HSA-437239
Reactome: R-HSA-5610780
Reactome: R-HSA-5632684
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Endosome Membrane
Localizes To Perinuclear Endosomes In An Aak1-Dependent Manner
Alternative Antibody Names Anti-Protein Numb Homolog antibody
Anti-H-Numb antibody
Anti-Protein S171 antibody
Anti-NUMB antibody
Anti-C14orf41 antibody

Information sourced from Uniprot.org

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