• Immunofluorescence analysis of U2OS cells using NUMA1 antibody (STJ24848) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using NUMA1 antibody (STJ24848) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using NUMA1 antibody (STJ24848) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using NUMA1 antibody (STJ24848) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NUMA1 antibody (STJ24848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NUMA1 antibody (1-309) (STJ24848)

SKU:
STJ24848

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUMA1 (1-309) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUMA1
Gene ID: 4926
Uniprot ID: NUMA1_HUMAN
Immunogen Region: 1-309
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human NUMA1 (NP_006176.2).
Immunogen Sequence: MTLHATRGAALLSWVNSLHV ADPVEAVLQLQDCSIFIKII DRIHGTEEGQQILKQPVSER LDFVCSFLQKNRKHPSSPEC LVSAQKVLEGSELELAKMTM LLLYHSTMSSKSPRDWEQFE YKIQAELAVILKFVLDHEDG LNLNEDLENFLQKAPVPSTC SSTFPEELSPPSHQAKREIR FLELQKVASSSSGNNFLSGS PASPMGDILQTPQFQMRRLK KQLADERSNRDELELELAE
Post Translational Modifications Phosphorylation and dephosphorylation on Thr-2055 regulates the extent of cortical NUMA1 and the dynein-dynactin complex localization during mitotic metaphase and anaphase. In metaphase, phosphorylation on Thr-2055 occurs in a kinase CDK1-dependent manner.this phosphorylation maintains low levels of cortical dynein-dynactin complex at metaphase, and hence proper spindle positioning. In anaphase, dephosphorylated on Thr-2055 by phosphatase PPP2CA.this dephosphorylation stimulates its membrane association and with the dynein-dynactin complex its enrichment at the cell cortex, and hence robust spindle elongation. Probably also phosphorylated on Thr-2015 and Ser-2087 by CDK1.these phosphorylations may regulate its cell cortex recruitment during metaphase and anaphase. Phosphorylated on Thr-1047, Ser-1769, Ser-1772, Ser-1789 and Ser-1834 by PLK1.these phosphorylations induce cortical dynein-dynactin complex dissociation from the NUMA1-GPSM2 complex and negatively regulates cortical dynein-dynactin complex localization. ADP-ribosylated by TNKS at the onset of mitosis.ADP-ribosylation is not required for its localization to spindle poles. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. Ubiquitinated with 'Lys-63'-linked polyubiquitin chains. Deubiquitination by the BRISC complex is important for the incorporation of NUMA1 into mitotic spindle poles and normal spindle pole function, probably by modulating interactions between NUMA1, dynein-dynactin complex and importin-beta.
Function Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division. Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles. Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner. In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle. During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation. Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro. Also required for proper orientation of the mitotic spindle during asymmetric cell divisions. Plays a role in mitotic MT aster assembly. Involved in anastral spindle assembly. Positively regulates TNKS protein localization to spindle poles in mitosis. Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume. Required for epidermal differentiation and hair follicle morphogenesis.
Protein Name Nuclear Mitotic Apparatus Protein 1
Nuclear Matrix Protein-22
Nmp-22
Nuclear Mitotic Apparatus Protein
Numa Protein
Sp-H Antigen
Database Links Reactome: R-HSA-380320
Reactome: R-HSA-68875
Cellular Localisation Nucleus
Nucleoplasm
Nucleus Matrix
Chromosome
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle Pole
Cell Cortex
Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Lateral Cell Membrane
Mitotic Cell Cycle-Dependent Shuttling Protein That Relocalizes From The Interphase Nucleus To The Spindle Poles And Cell Cortex
The Localization To The Spindle Poles Is Regulated By Aaas
In Interphase
Resides In The Nuclear Matrix
In Prophase
Restricted To The Interchromatin Or Condensed Chromosome Space
In Prometaphase
After Nuclear Envelope Disassembly
Forms Aggregates Both In The Spindle Midzone And At Duplicated Centrosomes And Astral Microtubules (Mts) Of The Bipolar Spindle Apparatus
Translocates From The Spindle Midzone Towards The Spindle Poles Along Spindle Fibers In A Mt- And Dynein-Dynactin-Dependent Manner Until The Anaphase Onset
In Metaphase
Recruited To The Polar Cortical Region In A Gpsm2- And Gnai1-Dependent Manner
Excluded From The Metaphase Equatorial Cortical Region In A Rangtp-Dependent Manner
Phosphorylation On Thr-2055 By Cdk1 Results In Its Localization At Spindle Poles In Metaphase
But Not At The Cell Cortex
In Anaphase
Recruited And Anchored At The Cell Membrane Of The Polar Cortical Region In A Epb41-
Epb41l2-
Phosphatidylinositol-Dependent And Gpsm2- And G(I) Alpha Proteins-Independent Manner
Excluded From The Anaphase Equatorial Region Of The Cell Cortex In A Racgap1- And Kif23-Dependent And Rangtp-Independent Manner
Associated With Astral Mts Emanating From The Spindle Poles During Anaphase
Nonphosphorylated Thr-2055 Localizes At The Cell Cortex
Weakly During Metaphase And More Prominently During Anaphase In A Phosphatase Ppp2ca-Dependent Manner
As Mitosis Progresses It Reassociates With Telophase Chromosomes Very Early During Nuclear Reformation
Before Substantial Accumulation Of Lamins On Chromosomal Surfaces Is Evident
Localizes To The Tips Of Cortical Mts In Prometaphase
Localizes Along Mts And Specifically To Both Mt Plus And Minus Ends
Accumulates Also At Mt Tips Near The Cell Periphery
Colocalizes With Gpsm2 At Mitotic Spindle Poles During Mitosis
Colocalizes With Spag5 At Mitotic Spindle At Prometaphase And At Mitotic Spindle Poles At Metaphase And Anaphase
Colocalizes With Abro1 At Mitotic Spindle Poles
Colocalized With Tnks From Prophase Through To Anaphase In Mitosis
Colocalizes With Tubulin Alpha
Ccsap Is Essential For Its Centrosomal Localization
In Horizontally Retinal Progenitor Dividing Cells
Localized To The Lateral Cortical Region
Isoform 3: Cytoplasm
Cytosol
During Interphase
Mainly Clustered At The Centrosomal Region In The Cytosol
After Entry Into Mitosis
Detected At Mitotic Spindle Poles
Isoform 4: Cytoplasm
Alternative Antibody Names Anti-Nuclear Mitotic Apparatus Protein 1 antibody
Anti-Nuclear Matrix Protein-22 antibody
Anti-Nmp-22 antibody
Anti-Nuclear Mitotic Apparatus Protein antibody
Anti-Numa Protein antibody
Anti-Sp-H Antigen antibody
Anti-NUMA1 antibody
Anti-NMP22 antibody
Anti-NUMA antibody

Information sourced from Uniprot.org

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