• Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NUDT21 antibody (25-75 aa) (STJ195715)

SKU:
STJ195715

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cleavage and polyadenylation specificity factor subunit 5 (25-75 aa) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUDT21
Gene ID: 11051
Uniprot ID: CPSF5_HUMAN
Immunogen Region: 25-75 aa
Specificity: This antibody detects endogenous levels of CPSF5 at Human/Mouse/Rat
Immunogen: Synthesized peptide derived from the human CPSF5 at the amino acid range 25-75
Post Translational Modifications Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 and SIRT2.
Function Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function. Binds to chromatin. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides.
Protein Name Cleavage And Polyadenylation Specificity Factor Subunit 5
Cleavage And Polyadenylation Specificity Factor 25 Kda Subunit
Cpsf 25 Kda Subunit
Cleavage Factor Im Complex 25 Kda Subunit
Cfim25
Nucleoside Diphosphate-Linked Moiety X Motif 21
Nudix Motif 21
Nudix Hydrolase 21
Pre-Mrna Cleavage Factor Im 68 Kda Subunit
Database Links Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Reactome: R-HSA-77595
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Nucleus And The Cytoplasm In A Transcription- And Xpo1/Crm1-Independent Manner
Most Probably In Complex With The Cleavage Factor Im Complex (Cfim)
In Punctate Subnuclear Structures Localized Adjacent To Nuclear Speckles
Called Paraspeckles
Alternative Antibody Names Anti-Cleavage And Polyadenylation Specificity Factor Subunit 5 antibody
Anti-Cleavage And Polyadenylation Specificity Factor 25 Kda Subunit antibody
Anti-Cpsf 25 Kda Subunit antibody
Anti-Cleavage Factor Im Complex 25 Kda Subunit antibody
Anti-Cfim25 antibody
Anti-Nucleoside Diphosphate-Linked Moiety X Motif 21 antibody
Anti-Nudix Motif 21 antibody
Anti-Nudix Hydrolase 21 antibody
Anti-Pre-Mrna Cleavage Factor Im 68 Kda Subunit antibody
Anti-NUDT21 antibody
Anti-CFIM25 antibody
Anti-CPSF25 antibody
Anti-CPSF5 antibody

Information sourced from Uniprot.org

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