| Tissue Specificity | Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Post Translational Modifications | Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 and SIRT2. |
| Function | Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function. Binds to chromatin. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. |
| Protein Name | Cleavage And Polyadenylation Specificity Factor Subunit 5Cleavage And Polyadenylation Specificity Factor 25 Kda SubunitCpsf 25 Kda SubunitCleavage Factor Im Complex 25 Kda SubunitCfim25Nucleoside Diphosphate-Linked Moiety X Motif 21Nudix Motif 21Nudix Hydrolase 21Pre-Mrna Cleavage Factor Im 68 Kda Subunit |
| Database Links | Reactome: R-HSA-72187Reactome: R-HSA-72203Reactome: R-HSA-73856Reactome: R-HSA-77595 |
| Cellular Localisation | NucleusCytoplasmShuttles Between The Nucleus And The Cytoplasm In A Transcription- And Xpo1/Crm1-Independent MannerMost Probably In Complex With The Cleavage Factor Im Complex (Cfim)In Punctate Subnuclear Structures Localized Adjacent To Nuclear SpecklesCalled Paraspeckles |
| Alternative Antibody Names | Anti-Cleavage And Polyadenylation Specificity Factor Subunit 5 antibodyAnti-Cleavage And Polyadenylation Specificity Factor 25 Kda Subunit antibodyAnti-Cpsf 25 Kda Subunit antibodyAnti-Cleavage Factor Im Complex 25 Kda Subunit antibodyAnti-Cfim25 antibodyAnti-Nucleoside Diphosphate-Linked Moiety X Motif 21 antibodyAnti-Nudix Motif 21 antibodyAnti-Nudix Hydrolase 21 antibodyAnti-Pre-Mrna Cleavage Factor Im 68 Kda Subunit antibodyAnti-NUDT21 antibodyAnti-CFIM25 antibodyAnti-CPSF25 antibodyAnti-CPSF5 antibody |
Information sourced from Uniprot.org
