| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NUDT16 |
| Gene ID: | 131870 |
| Uniprot ID: | NUD16_HUMAN |
| Immunogen Region: | 60-130 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-130 of human NUDT16 (NP_689608.2). |
| Immunogen Sequence: | GFVDTQDRSLEDGLNRELRE ELGEAAAAFRVERTDYRSSH VGSGPRVVAHFYAKRLTLEE LLAVEAGATRA |
| Tissue Specificity | Expressed strongly in lung, kidney, adrenal gland, testis, heart and brain. |
| Function | RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA.metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase.hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms. The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP. Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. Exhibits decapping activity towards NAD-capped RNAs and FAD-capped RNAs. Exhibits decapping activity towards dpCoA-capped RNAs in vitro. |
| Protein Name | U8 Snorna-Decapping Enzyme Idp Phosphatase Idpase Inosine Diphosphate Phosphatase Nucleoside Diphosphate-Linked Moiety X Motif 16 Nudix Motif 16 Nudix Hydrolase 16 U8 Snorna-Binding Protein H29k M7gpppn-Mrna Hydrolase |
| Database Links | Reactome: R-HSA-2393930 |
| Cellular Localisation | Nucleus Nucleoplasm Nucleolus Cytoplasm Localized Predominantly In The Cytoplasm Localized In Nucleolus And In A Minor Proportion In Distinct Foci In The Nucleoplasm |
| Alternative Antibody Names | Anti-U8 Snorna-Decapping Enzyme antibody Anti-Idp Phosphatase antibody Anti-Idpase antibody Anti-Inosine Diphosphate Phosphatase antibody Anti-Nucleoside Diphosphate-Linked Moiety X Motif 16 antibody Anti-Nudix Motif 16 antibody Anti-Nudix Hydrolase 16 antibody Anti-U8 Snorna-Binding Protein H29k antibody Anti-M7gpppn-Mrna Hydrolase antibody Anti-NUDT16 antibody |
Information sourced from Uniprot.org