| Host: | Goat |
| Applications: | Pep-ELISA/WB/IF |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-NUDC (Internal) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 40kDa band observed in lysates of cell lines HeLa. HepG2 and K562, and approx. 38kDa in lysates of cell line KNRK (calculated MW of 38.2kDa according to Human NP_006591.1) and 3 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | NUDC |
| Gene ID: | 10726 |
| Uniprot ID: | NUDC_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_006591.1 |
| Immunogen Sequence: | PNLGNGADLPNYR |
| Post Translational Modifications | Reversibly phosphorylated on serine residues during the M phase of the cell cycle. Phosphorylation on Ser-274 and Ser-326 is necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Phosphorylated by PLK and other kinases. |
| Function | Plays a role in neurogenesis and neuronal migration. Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation. |
| Protein Name | Nuclear Migration Protein NudcNuclear Distribution Protein C Homolog |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025Reactome: R-HSA-9696270 |
| Cellular Localisation | CytoplasmCytoskeletonNucleusSpindleMidbodyIn A Filamentous Pattern Adjacent To The Nucleus Of Migrating Cerebellar Granule CellsColocalizes With Tubulin And Dynein And With The Microtubule Organizing CenterDistributed Throughout The Cytoplasm Of Non-Migrating CellsA Small Proportion Is NuclearIn A Punctate PatternLocalizes To The Mitotic Spindle In A Eml4-Dependent Manner |
| Alternative Antibody Names | Anti-Nuclear Migration Protein Nudc antibodyAnti-Nuclear Distribution Protein C Homolog antibodyAnti-NUDC antibody |
Information sourced from Uniprot.org
