Anti-Nucleophosmin antibody [FC-61991] (STJ16100133)

SKU:
STJ16100133-100

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Host: Mouse
Applications: WB/IHC/ICC/IF/ELISA/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Nucleophosmin is suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: FC-61991
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS with 0.1% sodium azide
Concentration: 0.5 mg/mL
Dilution Range: ELISA 1-2 µg/mL
ICC
2-10 µg/mLIF 2-10 µg/mL
Storage Instruction: Store at 2-8°C upon receipt.
Gene Symbol: NPM1
Gene ID: 4869
Uniprot ID: NPM_HUMAN
Immunogen: NPM/B23 purified from rat hepatoma
Post Translational Modifications Acetylated at C-terminal lysine residues, thereby increasing affinity to histones. ADP-ribosylated. Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels. Sumoylated by ARF. Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36.
Function Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade. In complex with MYC enhances the transcription of MYC target genes. May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1.
Protein Name Nucleophosmin
Npm
Nucleolar Phosphoprotein B23
Nucleolar Protein No38
Numatrin
Database Links Reactome: R-HSA-180746
Reactome: R-HSA-3899300
Reactome: R-HSA-606279
Reactome: R-HSA-6804115
Reactome: R-HSA-8869496
Reactome: R-HSA-9692914
Reactome: R-HSA-9700645
Reactome: R-HSA-9725370
Reactome: R-HSA-9725371
Cellular Localisation Nucleus
Nucleolus
Nucleoplasm
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Generally Nucleolar
But Is Translocated To The Nucleoplasm In Case Of Serum Starvation Or Treatment With Anticancer Drugs
Has Been Found In The Cytoplasm In Patients With Primary Acute Myelogenous Leukemia (Aml)
But Not With Secondary Aml
Can Shuttle Between Cytoplasm And Nucleus
Co- Localizes With The Methylated Form Of Rps10 In The Granular Component (Gc) Region Of The Nucleolus
Colocalized With Nucleolin And Apex1 In Nucleoli
Isoform 1 Of Nek2 Is Required For Its Localization To The Centrosome During Mitosis
Alternative Antibody Names Anti-Nucleophosmin antibody
Anti-Npm antibody
Anti-Nucleolar Phosphoprotein B23 antibody
Anti-Nucleolar Protein No38 antibody
Anti-Numatrin antibody
Anti-NPM1 antibody
Anti-NPM antibody

Information sourced from Uniprot.org

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