| Short Description : | Rabbit polyclonal NTF4 (25-210) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:100-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NTF4 |
| Gene ID: | 4909 |
| Uniprot ID: | NTF4_HUMAN |
| Immunogen Region: | 25-210 |
| Immunogen Sequence: | QPPPSTLPPFLAPEWDLLSP RVVLSRGAPAGPPLLFLLEA GAFRESAGAPANRSRRGVSE TAPASRRGELAVCDAVSGWV TDRRTAVDLRGREVEVLGEV PAAGGSPLRQYFFETRCKAD NAEEGGPGAGGGGCRGVDRR HWVSECKAKQSYVRALTADA QGRVGWRWIRIDTACVCTLL SRTGRA |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-210 of human NTF4 (NP_006170.1). |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and STRADA knockout (KO) HeLa cells, using [KO Validated] STRADA Rabbit polyclonal antibody (STJ119985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98770/382819/STJ119985_1__92297.1713151316.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and STRADA knockout (KO) HeLa cells, using [KO Validated] STRADA Rabbit polyclonal antibody (STJ119985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
