• Immunofluorescence analysis of PC-3 cells using NTF4 Rabbit polyclonal antibody (STJ24831) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using NTF4 Rabbit polyclonal antibody (STJ24831) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-549 cells using NTF4 Rabbit polyclonal antibody (STJ24831) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of NTF4 in paraffin-embedded rat brain using NTF4 Rabbit polyclonal antibody (STJ24831) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NTF4 in paraffin-embedded human liver damage using NTF4 Rabbit polyclonal antibody (STJ24831) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using NTF4 Rabbit polyclonal antibody (STJ24831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-NTF4 antibody (25-210) (STJ24831)

SKU:
STJ24831

£112.50 - £524.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:100-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NTF4
Gene ID: 4909
Uniprot ID: NTF4_HUMAN
Immunogen Region: 25-210
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 25-210 of human NTF4 (NP_006170.1).
Immunogen Sequence: QPPPSTLPPFLAPEWDLLSP RVVLSRGAPAGPPLLFLLEA GAFRESAGAPANRSRRGVSE TAPASRRGELAVCDAVSGWV TDRRTAVDLRGREVEVLGEV PAAGGSPLRQYFFETRCKAD NAEEGGPGAGGGGCRGVDRR HWVSECKAKQSYVRALTADA QGRVGWRWIRIDTACVCTLL SRTGRA
Tissue Specificity Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.
Function Target-derived survival factor for peripheral sensory sympathetic neurons. May promote ameloblast differentiation and subsequent reduction in proliferation of ameloblasts.
Protein Name Neurotrophin-4
Nt-4
Neurotrophin-5
Nt-5
Neutrophic Factor 4
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-2219530
Reactome: R-HSA-6811558
Reactome: R-HSA-9026357
Reactome: R-HSA-9026519
Reactome: R-HSA-9026527
Reactome: R-HSA-9028335
Reactome: R-HSA-9028731
Cellular Localisation Secreted
Alternative Antibody Names Anti-Neurotrophin-4 antibody
Anti-Nt-4 antibody
Anti-Neurotrophin-5 antibody
Anti-Nt-5 antibody
Anti-Neutrophic Factor 4 antibody
Anti-NTF4 antibody
Anti-NTF5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance