| Host: | Rabbit |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NSUN6 |
| Gene ID: | 221078 |
| Uniprot ID: | NSUN6_HUMAN |
| Immunogen Region: | 220-469 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 220-469 of human NSUN6 (NP_872349.1). |
| Immunogen Sequence: | NLPSALVSHVLNPQPGEKIL DLCAAPGGKTTHIAALMHDQ GEVIALDKIFNKVEKIKQNA LLLGLNSIRAFCFDGTKAVK LDMVEDTEGEPPFLPESFDR ILLDAPCSGMGQRPNMACTW SVKEVASYQPLQRKLFTAAV QLLKPEGVLVYSTCTITLAE NEEQVAWALTKFPCLQLQPQ EPQIGGEGMRGAGLSCEQLK QLQRFDPSAVPLPDTDMDSL REARREDMLRLANKDSIGF |
| Function | S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA). In vitro also methylates tRNA(Thr)(AGT). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates. |
| Protein Name | Trna Cytosine(72-C(5-Methyltransferase Nsun6 Nol1/Nop2/Sun And Pua Domain-Containing Protein 1 Nol1/Nop2/Sun Domain Family Member 6 |
| Database Links | Reactome: R-HSA-6782315 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Trna antibody Anti-Cytosine(72-C(5-Methyltransferase Nsun6 antibody Anti-Nol1/Nop2/Sun And Pua Domain-Containing Protein 1 antibody Anti-Nol1/Nop2/Sun Domain Family Member 6 antibody Anti-NSUN6 antibody Anti-NOPD1 antibody |
Information sourced from Uniprot.org