• Western blot analysis of extracts from normal (control) and NSD3/WHSC1L1 knockout (KO) 293T cells, using NSD3/WHSC1L1 antibody (STJ28275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using NSD3/NSD3/WHSC1L1 antibody (STJ28275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NSD3 antibody (400-600) (STJ28275)

SKU:
STJ28275

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WHSC1L1 (400-600) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NSD3
Gene ID: 54904
Uniprot ID: NSD3_HUMAN
Immunogen Region: 400-600
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-600 of human NSD3/NSD3/WHSC1L1 (NP_060248.2).
Immunogen Sequence: EEALSQAKKSVASKTEVKKT RRPRSVLNTQPEQTNAGEVA SSLSSTEIRRHSQRRHTSAE EEEPPPVKIAWKTAAARKSL PASITMHKGSLDLQKCNMSP VVKIEQVFALQNATGDGKFI DQFVYSTKGIGNKTEISVRG QDRLIISTPNQRNEKPTQSV SSPEATSGSTGSVEKKQQRR SIRTRSESEKSTEVVPKKKI K
Tissue Specificity Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.
Function Histone methyltransferase. Preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3 forming H3K2me2 and H3K27me2. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.
Protein Name Histone-Lysine N-Methyltransferase Nsd3
Nuclear Set Domain-Containing Protein 3
Protein Whistle
Whsc1-Like 1 Isoform 9 With Methyltransferase Activity To Lysine
Wolf-Hirschhorn Syndrome Candidate 1-Like Protein 1
Whsc1-Like Protein 1
Database Links Reactome: R-HSA-3214841
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Nsd3 antibody
Anti-Nuclear Set Domain-Containing Protein 3 antibody
Anti-Protein Whistle antibody
Anti-Whsc1-Like 1 Isoform 9 With Methyltransferase Activity To Lysine antibody
Anti-Wolf-Hirschhorn Syndrome Candidate 1-Like Protein 1 antibody
Anti-Whsc1-Like Protein 1 antibody
Anti-NSD3 antibody
Anti-WHSC1L1 antibody
Anti-DC28 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance