• Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using NRF1 antibody (STJ11101843) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunohistochemistry analysis of NRF1 in paraffin-embedded human colon using NRF1 Rabbit monoclonal antibody (STJ11101843) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using NRF1 Rabbit monoclonal antibody (STJ11101843) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of various lysates using NRF1 Rabbit monoclonal antibody (STJ11101843) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ HepG2 cells with 1 Mu g NRF1 Rabbit monoclonal antibody (STJ11101843) , along with a Goat Anti-Rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of NRF1 in representative gene loci (LINS1) , as shown in figure.

Anti-NRF1 antibody (300-400) [S3MR] (STJ11101843)

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STJ11101843

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-NRF1 (300-400) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000 IHC-P 1:50-1:200
IP 1:50-1:100
ChIP 1:20-1:50
CUT&Tag 10⁵ cells/1 Mu g
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NRF1
Gene ID: 4899
Uniprot ID: NRF1_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human NRF1 (Q16656).
Immunogen Sequence: LVPSQTVVQTFSNPDGTVSL IQVGTGATVATLADASELPT TVTVAQVNYSAVADGEVEQN WATLQGGEMTIQTTQASEAT QAVASLAEAAVAASQEMQQG A
Tissue Specificity Ubiquitously expressed with strongest expression in skeletal muscle.
Post Translational Modifications Phosphorylation enhances DNA binding.
Function Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
Protein Name Nuclear Respiratory Factor 1
Nrf-1
Alpha Palindromic-Binding Protein
Alpha-Pal
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-2151201
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Respiratory Factor 1 antibody
Anti-Nrf-1 antibody
Anti-Alpha Palindromic-Binding Protein antibody
Anti-Alpha-Pal antibody
Anti-NRF1 antibody

Information sourced from Uniprot.org

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