• Confocal imaging of U-2 OS cells using KRAS+HRAS+NRAS Rabbit monoclonal antibody (STJ11102142, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using KRAS+HRAS+NRAS Rabbit monoclonal antibody (STJ11102142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from Rat lung, using KRAS+HRAS+NRAS Rabbit monoclonal antibody (STJ11102142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NRAS antibody (1-100) [S2MR] (STJ11102142)

SKU:
STJ11102142

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NRAS
HRAS
Gene ID: 4893
3265
Uniprot ID: RASN_HUMAN
RASH_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NRAS (P01111).
Immunogen Sequence: MTEYKLVVVGAGGVGKSALT IQLIQNHFVDEYDPTIEDSY RKQVVIDGETCLLDILDTAG QEEYSAMRDQYMRTGEGFLC VFAINNSKSFADINLYREQI
Tissue Specificity P01111 - NA
P01112 - Widely expressed.
Alternative Antibody Names Anti-Gtpase Nras antibody
Anti-Transforming Protein N-Ras antibody
Anti-NRAS antibody
Anti-HRAS1 antibody
Anti-Gtpase Hras antibody
Anti-H-Ras-1 antibody
Anti-Ha-Ras antibody
Anti-Transforming Protein P21 antibody
Anti-C-H-Ras antibody
Anti-P21ras Cleaved Into - Gtpase Hras - N-Terminally Processed antibody
Anti-HRAS antibody
Anti-HRAS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance