| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Nuclear Receptor Subfamily 1 Group D Member 2 (220-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4683RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NR1D2 |
| Gene ID: |
9975 |
| Uniprot ID: |
NR1D2_HUMAN |
| Immunogen Region: |
220-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 220-300 of human Rev-erb beta/NR1D2 (NP_005117.3). |
| Immunogen Sequence: |
ALPAQEQLRPKPQLEQENIK SSSPPSSDFAKEEVIGMVTR AHKDTFMYNQEQQENSAESM QPQRGERIPKNMEQYNLNHD H |
| Tissue Specificity | Widely expressed. Expressed at high levels in the liver, adipose tissue, skeletal muscle and brain. Expression oscillates diurnally in the suprachiasmatic nucleus (SCN) of the hypothalamus as well as in peripheral tissues. |
| Post Translational Modifications | Deacetylated by HDAC1. Acetylation and deacetylation regulate its transcriptional regulatory activity. Under more reducing intracellular redox conditions, Cys-384 is in its heme-bound state, which is optimal for recruitment of the NCOR1/HDAC3 corepressor complex and repression of target genes. When subjected to oxidative stress conditions, Cys-384 undergoes oxidation to form a disulfide bridge with Cys-374, also triggering a ligand switch that results in release of bound heme and derepression of target genes. Ubiquitinated by SIAH2.leading to proteasomal degradation. Phosphorylated by CSNK1E.phosphorylation enhances its cytoplasmic localization. |
| Function | Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components BMAL1 and CLOCK. Also regulates genes involved in metabolic functions, including lipid metabolism and the inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5'-A/GGGTCA-3' preceded by an A/T-rich 5' sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two nuclegotides (RevDR-2). Acts as a potent competitive repressor of ROR alpha (RORA) function and also negatively regulates the expression of NR1D1. Regulates lipid and energy homeostasis in the skeletal muscle via repression of genes involved in lipid metabolism and myogenesis including: CD36, FABP3, FABP4, UCP3, SCD1 and MSTN. Regulates hepatic lipid metabolism via the repression of APOC3. Represses gene expression at a distance in macrophages by inhibiting the transcription of enhancer-derived RNAs (eRNAs). In addition to its activity as a repressor, can also act as a transcriptional activator. Acts as a transcriptional activator of the sterol regulatory element-binding protein 1 (SREBF1) and the inflammatory mediator interleukin-6 (IL6) in the skeletal muscle. Plays a role in the regulation of circadian sleep/wake cycle.essential for maintaining wakefulness during the dark phase or active period. Key regulator of skeletal muscle mitochondrial function.negatively regulates the skeletal muscle expression of core clock genes and genes involved in mitochondrial biogenesis, fatty acid beta-oxidation and lipid metabolism. May play a role in the circadian control of neutrophilic inflammation in the lung. |
| Protein Name | Nuclear Receptor Subfamily 1 Group D Member 2Orphan Nuclear Hormone Receptor Bd73Rev-Erb Alpha-Related ReceptorRvrRev-Erb-BetaV-Erba-Related Protein 1-RelatedEar-1r |
| Database Links | Reactome: R-HSA-383280 |
| Cellular Localisation | NucleusCytoplasmPhosphorylation By Csnk1e Enhances Its Cytoplasmic Localization |
| Alternative Antibody Names | Anti-Nuclear Receptor Subfamily 1 Group D Member 2 antibodyAnti-Orphan Nuclear Hormone Receptor Bd73 antibodyAnti-Rev-Erb Alpha-Related Receptor antibodyAnti-Rvr antibodyAnti-Rev-Erb-Beta antibodyAnti-V-Erba-Related Protein 1-Related antibodyAnti-Ear-1r antibodyAnti-NR1D2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
