Anti-NQO1 antibody (1-274) (STJ24805)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NQO1 (1-274) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NQO1 |
| Gene ID | 1728 |
| Uniprot ID | NQO1_HUMAN |
| Immunogen Region | 1-274 |
| Immunogen Sequence | MVGRRALIVLAHSERTSFNY AMKEAAAAALKKKGWEVVES DLYAMNFNPIISRKDITGKL KDPANFQYPAESVLAYKEGH LSPDIVAEQKKLEAADLVIF QFPLQWFGVPAILKGWFERV FIGEFAYTYAAMYDKGPFRS KKAVLSITTGGSGSMYSLQG IHGDMNVILWPIQSGILHFC GFQVLEPQLTYSIGHTPADA RIQILEGWKKRLENIWDETP LYFAPSSLFDLNFQAGFLM |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human NQO1 (NP_000894.1). |
Additional Info
| Function | Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species. Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion. Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential. Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome. |
| Protein Name | Nad(Ph Dehydrogenase Quinone 1AzoreductaseDt-DiaphoraseDtdMenadione ReductaseNad(Ph -Quinone Oxidoreductase 1Phylloquinone ReductaseQuinone Reductase 1Qr1 |
| Database Links | Reactome: R-HSA-350562Reactome: R-HSA-9818027 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Nad(Ph Dehydrogenase Quinone 1 antibodyAnti-Azoreductase antibodyAnti-Dt-Diaphorase antibodyAnti-Dtd antibodyAnti-Menadione Reductase antibodyAnti-Nad(Ph -Quinone Oxidoreductase 1 antibodyAnti-Phylloquinone Reductase antibodyAnti-Quinone Reductase 1 antibodyAnti-Qr1 antibodyAnti-NQO1 antibodyAnti-DIA4 antibodyAnti-NMOR1 antibody |
Information sourced from Uniprot.org