| Short Description : | Rabbit polyclonal NPLOC4 (1-270) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:1000-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NPLOC4 |
| Gene ID: | 55666 |
| Uniprot ID: | NPL4_HUMAN |
| Immunogen Region: | 1-270 |
| Immunogen Sequence: | MAESIIIRVQSPDGVKRITA TKRETAATFLKKVAKEFGFQ NNGFSVYINRNKTGEITASS NKSLNLLKIKHGDLLFLFPS SLAGPSSEMETSVPPGFKVF GAPNVVEDEIDQYLSKQDGK IYRSRDPQLCRHGPLGKCVH CVPLEPFDEDYLNHLEPPVK HMSFHAYIRKLTGGADKGKF VALENISCKIKSGCEGHLPW PNGICTKCQPSAITLNRQKY RHVDNIMFENHTVADRFLD |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NPLOC4 (NP_060391.2). |
| Tissue Specificity | Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver. |
| Function | The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to RIGI and recruits RNF125 to promote ubiquitination and degradation of RIGI. |
| Protein Name | Nuclear Protein Localization Protein 4 HomologProtein Npl4 |
| Database Links | Reactome: R-HSA-110320Reactome: R-HSA-8951664Reactome: R-HSA-9755511 |
| Cellular Localisation | CytoplasmCytosolEndoplasmic ReticulumNucleusAssociated With The Endoplasmic Reticulum And Nuclear |
| Alternative Antibody Names | Anti-Nuclear Protein Localization Protein 4 Homolog antibodyAnti-Protein Npl4 antibodyAnti-NPLOC4 antibodyAnti-KIAA1499 antibodyAnti-NPL4 antibody |
Information sourced from Uniprot.org
