| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal anti-Nephrin (843-892) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NPHS1 |
| Gene ID: | 4868 |
| Uniprot ID: | NPHN_HUMAN |
| Immunogen Region: | 843-892 |
| Specificity: | Nephrin Polyclonal Antibody detects endogenous levels of Nephrin protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Nephrin. AA range:843-892 |
| Tissue Specificity | Specifically expressed in podocytes of kidney glomeruli. |
| Post Translational Modifications | Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. Tyrosine phosphorylation is reduced by high glucose levels. Dephosphorylated by tensin TNS2 which leads to reduced binding of NPHN1 to PIK3R1. |
| Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. |
| Protein Name | NephrinRenal Glomerulus-Specific Cell Adhesion Receptor |
| Database Links | Reactome: R-HSA-373753 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinPredominantly Located At Podocyte Slit Diaphragm Between Podocyte Foot ProcessesAlso Associated With Podocyte Apical Plasma Membrane |
| Alternative Antibody Names | Anti-Nephrin antibodyAnti-Renal Glomerulus-Specific Cell Adhesion Receptor antibodyAnti-NPHS1 antibodyAnti-NPHN antibody |
Information sourced from Uniprot.org
