• Western blot analysis of extracts of mouse pancreas, using NPHP1 antibody (STJ28757) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using NPHP1 antibody (STJ28757) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using NPHP1 rabbit polyclonal antibody (STJ28757) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using NPHP1 rabbit polyclonal antibody (STJ28757) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using NPHP1 rabbit polyclonal antibody (STJ28757) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using NPHP1 rabbit polyclonal antibody (STJ28757) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using NPHP1 rabbit polyclonal antibody (STJ28757) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NPHP1 antibody (463-732) (STJ28757)

SKU:
STJ28757

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NPHP1 (463-732) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NPHP1
Gene ID: 4867
Uniprot ID: NPHP1_HUMAN
Immunogen Region: 463-732
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 463-732 of human NPHP1 (NP_997064.2).
Immunogen Sequence: PDLGILFELGISYIRNSTGE RGELSCGWVFLKLFDASGVP IPAKTYELFLNGGTPYEKGI EVDPSISRRAHGSVFYQIMT MRRQPQLLVKLRSLNRRSRN VLSLLPETLIGNMCSIHLLI FYRQILGDVLLKDRMSLQST DLISHPMLATFPMLLEQPDV MDALRSSWAGKESTLKRSEK RDKEFLKSTFLLVYHDCVLP LLHSTRLPPFRWAEEETETA RWKVITDFLKQNQENQGAL
Tissue Specificity Widespread expression, with highest levels in pituitary gland, spinal cord, thyroid gland, testis, skeletal muscle, lymph node and trachea. Weakly expressed in heart, kidney and pancreas. Expressed in nasal epithelial cells (at protein level). Expressed in the renal collecting duct (at protein level).
Post Translational Modifications Phosphorylation by CK2 is required for the interaction with PACS1 and the targeting to the base region of cilia.
Function Together with BCAR1 it may play a role in the control of epithelial cell polarity. Involved in the organization of apical junctions in kidney cells together with NPHP4 and RPGRIP1L/NPHP8. Does not seem to be strictly required for ciliogenesis. Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling. May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly. Required for normal retina development. In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19. Involved in spermatogenesis.
Protein Name Nephrocystin-1
Juvenile Nephronophthisis 1 Protein
Database Links Reactome: R-HSA-5620912
Cellular Localisation Cell Junction
Adherens Junction
Cell Projection
Cilium
Cytoplasm
Cytoskeleton
Cilium Axoneme
Tight Junction
In The Retinal Photoreceptor Cell Layer
Localizes At The Connecting Cilium
Colocalizes With E-Cadherin And Bcar1 At Or Near The Cell-Cell Adherens Junctions
Localized To Respiratory Cilia Axoneme
Localized To The Transition Zone Of Respiratory Cilia
Localized To The Transition Zone Of Photoreceptor-Connecting Cilia And Renal Monocilia
In Cultured Renal Cells
It Localizes Diffusely In The Cytoplasm But
As Cells Approach Confluence
It Accumulates At Basolateral Tight Junctions
Alternative Antibody Names Anti-Nephrocystin-1 antibody
Anti-Juvenile Nephronophthisis 1 Protein antibody
Anti-NPHP1 antibody
Anti-NPH1 antibody

Information sourced from Uniprot.org

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