• STJ72450 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing staining within the cytoplasm and plasma membrane. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72450 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing staining within the cytoplasm and plasma membrane. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72450 (0. 5µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-NOX5 antibody (Internal) (STJ72450)

SKU:
STJ72450-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/WB
Reactivity: Human/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-NOX5 (Internal) is suitable for use in ELISA, Immunofluorescence and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.5-2µg/ml
IF-Strong expression of the protein seen in the plasma membrane and cytoplasm of HeLa and MCF7 cells. Recommended concentration:10ug/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NOX5
Gene ID: 79400
Uniprot ID: NOX5_HUMAN
Immunogen Region: Internal
Accession Number: NP_078781.3; NP_001171708.1; NP_001171709.1
Specificity: This antibody is expected to recognize all reported isoforms (NP_078781.3; NP_001171708.1; NP_001171709.1).
Immunogen Sequence: EWHPFTISSAPEQKD
Post Translational Modifications Isoform v2: Phosphorylation at Ser-475 by CaMK2 and at Ser-490, Thr-494 and Ser-498 by PKC/PRKCA positively regulates its catalytic activity. Isoform v2: S-nitrosylation in response to nitric oxide inhibits its catalytic activity.
Function Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. May play a role in cell growth and apoptosis. Isoform v2: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. Involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. Isoform v1: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Isoform v5: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. lacks enzyme activity. Involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. Isoform v4: Lacks calcium-dependent NADPH oxidase activity. Isoform v3: Lacks calcium-dependent NADPH oxidase activity.
Protein Name Nadph Oxidase 5
Database Links Reactome: R-HSA-3299685
Cellular Localisation Isoform V2: Endoplasmic Reticulum
Cell Membrane
Multi-Pass Membrane Protein
Calcium-Sensitive Association And Dissociation Between The N- And C-Terminal Domains Appears To Facilitate Its Localization To The Cell Membrane
Isoform V5: Endoplasmic Reticulum
Alternative Antibody Names Anti-Nadph Oxidase 5 antibody
Anti-NOX5 antibody

Information sourced from Uniprot.org

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