| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Nadph Oxidase 4 (328-578) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NOX4 |
| Gene ID: |
50507 |
| Uniprot ID: |
NOX4_HUMAN |
| Immunogen Region: |
328-578 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 328-578 of human NADPH oxidase 4 (NOX4) (NP_058627.1). |
| Immunogen Sequence: |
KENFKARPGQYITLHCPSVS ALENHPFTLTMCPTETKATF GVHLKIVGDWTERFRDLLLP PSSQDSEILPFIQSRNYPKL YIDGPFGSPFEESLNYEVSL CVAGGIGVTPFASILNTLLD DWKPYKLRRLYFIWVCRDIQ SFRWFADLLCMLHNKFWQEN RPDYVNIQLYLSQTDGIQKI IGEKYHALNSRLFIGRPRWK LLFDEIAKYNRGKTVGVFCC GPNSLSKTLHKLSNQNNSY |
| Tissue Specificity | Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells. |
| Post Translational Modifications | Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function. |
| Function | Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity. Isoform 4: Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1. |
| Protein Name | Nadph Oxidase 4Kidney Oxidase-1Kox-1Kidney Superoxide-Producing Nadph OxidaseRenal Nad(Ph-Oxidase |
| Database Links | Reactome: R-HSA-3299685Reactome: R-HSA-9702518Reactome: R-HSA-9703465 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinCell MembraneCell JunctionFocal AdhesionMay Localize To Plasma Membrane And Focal AdhesionsMay Also Localize To The NucleusIsoform 4: NucleusNucleusNucleolus |
| Alternative Antibody Names | Anti-Nadph Oxidase 4 antibodyAnti-Kidney Oxidase-1 antibodyAnti-Kox-1 antibodyAnti-Kidney Superoxide-Producing Nadph Oxidase antibodyAnti-Renal Nad(Ph-Oxidase antibodyAnti-NOX4 antibodyAnti-RENOX antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
