Western blot analysis of various lysates using NOX1 Rabbit pAb (STJ111270) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-NOX1 antibody (415-564) (STJ111270)

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STJ111270
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Applications:WB/ELISA
Host:Rabbit
Reactivity:Human/Mouse/Rat
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:NOX1
Gene ID:27035
Uniprot ID:NOX1_HUMAN
Immunogen Region:415-564
Immunogen Sequence:KSIWYKFQCADHNLKTKKIY FYWICRETGAFSWFNNLLTS LEQEMEELGKVGFLNYRLFL TGWDSNIVGHAALNFDKATD IVTGLKQKTSFGRPMWDNEF STIATSHPKSVVGVFLCGPR TLAKSLRKCCHRYSSLDPRK VQFYFNKENF
Specificity:Recombinant fusion protein containing a sequence corresponding to amino acids 415-564 of human NOX1 (NP_008983.2).
Tissue Specificity Isoform NOH-1L: Detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes.
Post Translational Modifications Phosphorylation at Thr-430 mediated by PKC/PRKBC positively regulates its interaction with NOXA1 and enzyme activity.
Function NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Isoform NOH-1L: NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor.
Protein Name Nadph Oxidase 1
Nox-1
Mitogenic Oxidase 1
Mox-1
Nadh/Nadph Mitogenic Oxidase Subunit P65-Mox
Noh-1
Database Links Reactome: R-HSA-5668599
Reactome: R-HSA-9013149
Reactome: R-HSA-9013423
Reactome: R-HSA-9673324
Cellular Localisation Cell Projection
Invadopodium Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Nadph Oxidase 1 antibody
Anti-Nox-1 antibody
Anti-Mitogenic Oxidase 1 antibody
Anti-Mox-1 antibody
Anti-Nadh/Nadph Mitogenic Oxidase Subunit P65-Mox antibody
Anti-Noh-1 antibody
Anti-NOX1 antibody
Anti-MOX1 antibody
Anti-NOH1 antibody

Information sourced from Uniprot.org

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