| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NOTCH4 (1824-2003) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NOTCH4 |
| Gene ID: |
4855 |
| Uniprot ID: |
NOTC4_HUMAN |
| Immunogen Region: |
1824-2003 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1824-2003 of human NOTCH4 (NP_004548.3). |
| Immunogen Sequence: |
PPEARHKATPGREAGPFPRA RTVSVSVPPHGGGALPRCRT LSAGAGPRGGGACLQARTWS VDLAARGGGAYSHCRSLSGV GAGGGPTPRGRRFSAGMRGP RPNPAIMRGRYGVAAGRGGR VSTDDWPCDWVALGACGSAS NIPIPPPCLTPSPERGSPQL DCGPPALQEMPINQGGEGKK |
| Tissue Specificity | Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes. |
| Post Translational Modifications | Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. |
| Function | Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system. |
| Protein Name | Neurogenic Locus Notch Homolog Protein 4Notch 4Hnotch4 Cleaved Into - Notch 4 Extracellular Truncation - Notch 4 Intracellular Domain |
| Database Links | Reactome: R-HSA-1912399Reactome: R-HSA-1912408Reactome: R-HSA-1912420Reactome: R-HSA-350054Reactome: R-HSA-5083630Reactome: R-HSA-9013695Reactome: R-HSA-9013700Reactome: R-HSA-9604323 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinNotch 4 Intracellular Domain: NucleusFollowing Proteolytical Processing Nicd Is Translocated To The Nucleus |
| Alternative Antibody Names | Anti-Neurogenic Locus Notch Homolog Protein 4 antibodyAnti-Notch 4 antibodyAnti-Hnotch4 Cleaved Into - Notch 4 Extracellular Truncation - Notch 4 Intracellular Domain antibodyAnti-NOTCH4 antibodyAnti-INT3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
