| Host: |
Goat |
| Applications: |
Pep-ELISA/IF |
| Reactivity: |
Human/Mouse/Rat/Dog/Pig/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-NOTCH4 (1261-1273) is suitable for use in ELISA and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
IF-Strong expression of the protein seen in the nuclei and membranes of HeLa cells, and in the membranes of NIH3T3 cells. 10µg/mlELISA-antibody detection limit dilution 1:8000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
NOTCH4 |
| Gene ID: |
4855 |
| Uniprot ID: |
NOTC4_HUMAN |
| Immunogen Region: |
1261-1273 |
| Accession Number: |
NP_004548.3 |
| Specificity: |
The immunizong peptide represents part of the extracellular domain. |
| Immunogen Sequence: |
DHFHNGHCEKGCNN |
| Post Translational Modifications | Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. |
| Function | Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system. |
| Protein Name | Neurogenic Locus Notch Homolog Protein 4Notch 4Hnotch4 Cleaved Into - Notch 4 Extracellular Truncation - Notch 4 Intracellular Domain |
| Database Links | Reactome: R-HSA-1912399Reactome: R-HSA-1912408Reactome: R-HSA-1912420Reactome: R-HSA-350054Reactome: R-HSA-5083630Reactome: R-HSA-9013695Reactome: R-HSA-9013700Reactome: R-HSA-9604323 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinNotch 4 Intracellular Domain: NucleusFollowing Proteolytical Processing Nicd Is Translocated To The Nucleus |
| Alternative Antibody Names | Anti-Neurogenic Locus Notch Homolog Protein 4 antibodyAnti-Notch 4 antibodyAnti-Hnotch4 Cleaved Into - Notch 4 Extracellular Truncation - Notch 4 Intracellular Domain antibodyAnti-NOTCH4 antibodyAnti-INT3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
