• Western blot analysis of lysates from wild type (WT) and NOSIP knockout (KO) 293T cells using [KO Validated] NOSIP Rabbit polyclonal antibody (STJ11104394) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NOSIP antibody (1-301) (STJ11104394)

SKU:
STJ11104394

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nitric Oxide Synthase-Interacting Protein (1-301) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOSIP
Gene ID: 51070
Uniprot ID: NOSIP_HUMAN
Immunogen Region: 1-301
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 1-301 of human NOSIP (NP_057037.1).
Immunogen Sequence: MTRHGKNCTAGAVYTYHEKK KDTAASGYGTQNIRLSRDAV KDFDCCCLSLQPCHDPVVTP DGYLYEREAILEYILHQKKE IARQMKAYEKQRGTRREEQK ELQRAASQDHVRGFLEKESA IVSRPLNPFTAKALSGTSPD DVQPGPSVGPPSKDKDKVLP SFWIPSLTPEAKATKLEKPS RTVTCPMSGKPLRMSDLTPV HFTPLDSSVDRVGLITRSER YVCAVTRDSLSNATPCAVL
Tissue Specificity Expressed in heart, brain and lung. Present in endothelial cells (at protein level).
Function E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB. Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity.
Protein Name Nitric Oxide Synthase-Interacting Protein
E3 Ubiquitin-Protein Ligase Nosip
Ring-Type E3 Ubiquitin Transferase Nosip
Enos-Interacting Protein
Database Links Reactome: R-HSA-203754
Cellular Localisation Cytoplasm
Nucleus
Translocates From Nucleus To Cytoplasm In The G2 Phase Of The Cell Cycle
Alternative Antibody Names Anti-Nitric Oxide Synthase-Interacting Protein antibody
Anti-E3 Ubiquitin-Protein Ligase Nosip antibody
Anti-Ring-Type E3 Ubiquitin Transferase Nosip antibody
Anti-Enos-Interacting Protein antibody
Anti-NOSIP antibody
Anti-CGI-25 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance