| Host: |
Mouse |
| Applications: |
WB/ICC/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-eNOS (C-Term) is suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M221 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHC 1:200ICC 1:50IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Nos3 |
| Gene ID: |
18127 |
| Uniprot ID: |
NOS3_MOUSE |
| Immunogen Region: |
C-Term |
| Specificity: |
The antibody detects a 140 kDa* protein corresponding to eNOS on SDS-PAGE immunoblots of human umbilical vein endothelial cells. |
| Immunogen: |
Clone (M221) was generated from a recombinant mouse eNOS protein that included amino acids residues in the C-terminal region. This sequence is conserved in human and rat eNOS, and has low homology to other NOS family members. |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development. |
| Protein Name | Nitric Oxide Synthase 3Constitutive NosCnosEc-NosNos Type IiiNosiiiNitric Oxide Synthase - EndothelialEndothelial NosEnos |
| Database Links | Reactome: R-MMU-1222556Reactome: -MMU-1474151Reactome: -MMU-203615Reactome: -MMU-203641Reactome: -MMU-203754Reactome: -MMU-392154Reactome: -MMU-5218920Reactome: -MMU-9009391 |
| Cellular Localisation | MembraneCaveolaCytoplasmCytoskeletonGolgi ApparatusCell MembraneSpecifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell CycleWhich Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity |
| Alternative Antibody Names | Anti-Nitric Oxide Synthase 3 antibodyAnti-Constitutive Nos antibodyAnti-Cnos antibodyAnti-Ec-Nos antibodyAnti-Nos Type Iii antibodyAnti-Nosiii antibodyAnti-Nitric Oxide Synthase - Endothelial antibodyAnti-Endothelial Nos antibodyAnti-Enos antibodyAnti-Nos3 antibodyAnti-Ecnos antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
