Anti-NOS2 antibody (117-166) (STJ94534)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human iNOS. AA range:117-166
General Information
| Short Description | Rabbit polyclonal anti-Nitric oxide synthase, inducible (117-166) for use in IF, WB, IHC and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/WB/IHC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IF 1:50-200WB 1:500-2000 ELISA 1:10000-20000IHC 1:50-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NOS2 |
| Gene ID | 4843 |
| Uniprot ID | NOS2_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from human iNOS. AA range:117-166 |
| Immunogen Region | 117-166 |
| Specificity | NOS2 Polyclonal Antibody detects endogenous levels of NOS2 protein. |
Additional Info
| Tissue Specificity | Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Expressed in chondrocytes. |
| Post Translational Modifications | Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation. |
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8. |
| Protein Name | Nitric Oxide Synthase - InducibleHepatocyte NosHep-NosInducible No SynthaseInducible NosInosNos Type IiPeptidyl-Cysteine S-Nitrosylase Nos2 |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-392154Reactome: R-HSA-6785807Reactome: R-HSA-9033241Reactome: R-HSA-9636249 |
| Cellular Localisation | CytoplasmCytosolLocalizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4Exhibits A More Diffuse Cytosolic Localization |
| Alternative Antibody Names | Anti-Nitric Oxide Synthase - Inducible antibodyAnti-Hepatocyte Nos antibodyAnti-Hep-Nos antibodyAnti-Inducible No Synthase antibodyAnti-Inducible Nos antibodyAnti-Inos antibodyAnti-Nos Type Ii antibodyAnti-Peptidyl-Cysteine S-Nitrosylase Nos2 antibodyAnti-NOS2 antibodyAnti-NOS2A antibody |
Information sourced from Uniprot.org