• Western blot analysis of 3T3 lysis using NOS2 antibody. Antibody was diluted at 1:500
  • Western blot analysis of lysates from NIH/3T3 cells, using iNOS Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of 3T3 lysis using NOS2 antibody. Antibody was diluted at 1:500
  • Western blot analysis of 3T3 lysis using NOS2 antibody. Antibody was diluted at 1:500
  • Western blot analysis of various cells using NOS2 Polyclonal Antibody diluted at 1：500
  • Western blot analysis of SH-SY5Y cells using NOS2 Polyclonal Antibody diluted at 1：500
  • Immunofluorescence analysis of mouse-kidney tissue. 1, NOS2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, NOS2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of mouse-kidney tissue. 1, NOS2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of mouse-lung tissue. 1, NOS2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of mouse-lung tissue. 1, NOS2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-kidney tissue. 1, NOS2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-kidney tissue. 1, NOS2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-NOS2 antibody (117-166) (STJ94534)

SKU:
STJ94534

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IF/WB/IHC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nitric oxide synthase, inducible (117-166) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:500-2000 ELISA 1:10000-20000
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOS2
Gene ID: 4843
Uniprot ID: NOS2_HUMAN
Immunogen Region: 117-166
Specificity: NOS2 Polyclonal Antibody detects endogenous levels of NOS2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human iNOS. AA range:117-166
Tissue Specificity Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Expressed in chondrocytes.
Post Translational Modifications Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation.
Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8.
Protein Name Nitric Oxide Synthase - Inducible
Hepatocyte Nos
Hep-Nos
Inducible No Synthase
Inducible Nos
Inos
Nos Type Ii
Peptidyl-Cysteine S-Nitrosylase Nos2
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-392154
Reactome: R-HSA-6785807
Reactome: R-HSA-9033241
Reactome: R-HSA-9636249
Cellular Localisation Cytoplasm
Cytosol
Localizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4
Exhibits A More Diffuse Cytosolic Localization
Alternative Antibody Names Anti-Nitric Oxide Synthase - Inducible antibody
Anti-Hepatocyte Nos antibody
Anti-Hep-Nos antibody
Anti-Inducible No Synthase antibody
Anti-Inducible Nos antibody
Anti-Inos antibody
Anti-Nos Type Ii antibody
Anti-Peptidyl-Cysteine S-Nitrosylase Nos2 antibody
Anti-NOS2 antibody
Anti-NOS2A antibody

Information sourced from Uniprot.org

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