• Western blot of HeLa cell lysate showing specific immunolabeling of the ~34 kDa fibrillarin protein.
  • High magnification confocal image of HeLa cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin (STJA0003634, 1:100, green) , colabeled with Anti-Vimentin (STJA0003823, red, 1:1000) showing the cytoplasmic intermediated filaments, and nuclear staining was done with DAPI (blue).
  • Immunofluorescence of human neuroblastoma SH-SY5Y cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin (STJA0003634, 1:500, green) , and nuclear staining was done with DAPI (blue) which makes the fibrillarin marker appear pale blue.

Anti-NOP1 antibody [38F3] (STJA0003634)

SKU:
STJA0003634-100

£403.50
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Host: Mouse
Applications: WB/IHC/ICC
Reactivity: Bovine/Chicken/D.melanogaster/Human/Mouse/Pig/Rat/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Vole
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Fibrillarin is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: 38F3
Conjugation: Unconjugated
Isotype: IgG1
Formulation: 100 ul in PBS + 50% Glycerol and 5 mm Sodium Azide
Purification: This antibody was protein g purified culture from supernatant.
Dilution Range: WB 1:1000
IHC 1:100-1:500
ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: Yeast nuclear preparations.
Background Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa) , is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although Western blot of HeLa cell lysate showing specific mice with only one functional fibrillarin/Nop1p gene were viable immunolabeling of the ~34 kDa fibrillarin protein. (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Information sourced from Uniprot.org

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