| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NONO (1-471) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NONO |
| Gene ID: |
4841 |
| Uniprot ID: |
NONO_HUMAN |
| Immunogen Region: |
1-471 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-471 of human NONO/p54nrb (NP_001138881.1). |
| Immunogen Sequence: |
MQSNKTFNLEKQNHTPRKHH QHHHQQQHHQQQQQQPPPPP IPANGQQASSQNEGLTIDLK NFRKPGEKTFTQRSRLFVGN LPPDITEEEMRKLFEKYGKA GEVFIHKDKGFGFIRLETRT LAEIAKVELDNMPLRGKQLR VRFACHSASLTVRNLPQYVS NELLEEAFSVFGQVERAVVI VDDRGRPSGKGIVEFSGKPA ARKALDRCSEGSFLLTTFPR PVTVEPMDQLDDEEGLPEK |
| Tissue Specificity | Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines. |
| Post Translational Modifications | The N-terminus is blocked. |
| Function | DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Important for the functional organization of GABAergic synapses. Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript. Plays a key role during neuronal differentiation by recruiting TET1 to genomic loci and thereby regulating 5-hydroxymethylcytosine levels. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. Promotes activation of the cGAS-STING pathway in response to HIV-2 infection: acts by interacting with HIV-2 Capsid protein p24, thereby promoting detection of viral DNA by CGAS, leading to CGAS-mediated inmmune activation. In contrast, the weak interaction with HIV-1 Capsid protein p24 does not allow activation of the cGAS-STING pathway. |
| Protein Name | Non-Pou Domain-Containing Octamer-Binding ProteinNono Protein54 Kda Nuclear Rna- And Dna-Binding ProteinP54(NrbP54nrb55 Kda Nuclear ProteinNmt55Dna-Binding P52/P100 Complex - 52 Kda Subunit |
| Cellular Localisation | NucleusNucleolusNucleus SpeckleChromosomeDetected In Punctate Subnuclear Structures Often Located Adjacent To Splicing SpecklesCalled Paraspeckles |
| Alternative Antibody Names | Anti-Non-Pou Domain-Containing Octamer-Binding Protein antibodyAnti-Nono Protein antibodyAnti-54 Kda Nuclear Rna- And Dna-Binding Protein antibodyAnti-P54(Nrb antibodyAnti-P54nrb antibodyAnti-55 Kda Nuclear Protein antibodyAnti-Nmt55 antibodyAnti-Dna-Binding P52/P100 Complex - 52 Kda Subunit antibodyAnti-NONO antibodyAnti-NRB54 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
