• Western blot analysis of various lysates using NOL12 Rabbit polyclonal antibody (STJ116504) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NOL12 antibody (1-213) (STJ116504)

SKU:
STJ116504

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOL12
Gene ID: 79159
Uniprot ID: NOL12_HUMAN
Immunogen Region: 1-213
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human NOL12 (NP_077289.1).
Immunogen Sequence: MGRNKKKKRDGDDRRPRLVL SFDEEKRREYLTGFHKRKVE RKKAAIEEIKQRLKEEQRKL REERHQEYLKMLAEREEALE EADELDRLVTAKTESVQYDH PNHTVTVTTISDLDLSGARL LGLTPPEGGAGDRSEEEASS TEKPTKALPRKSRDPLLSQR ISSLTASLHAHSRKKVKRKH PRRAQDSKKPPRAPRTSKAQ RRRLTGKARHSGE
Function Multifunctional RNA binding protein that plays a role in RNA metabolism and DNA maintenance. Participates in the resolution of DNA stress and the maintenance of genome integrity by localizing to sites of DNA insults. Plays also a role in proper nucleolar organization by limiting nucleolar size and regulating nucleolar number. Mechanistically, regulates the nucleolar levels of fibrillarin and nucleolin, two key players in pre-rRNA processing and ribosome assembly.
Protein Name Nucleolar Protein 12
Database Links Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Alternative Antibody Names Anti-Nucleolar Protein 12 antibody
Anti-NOL12 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance