• Western blot analysis of extracts of various cell lines, using NODAL antibody (STJ111943) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using NODAL antibody (STJ111943) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NODAL antibody (198-347) (STJ111943)

SKU:
STJ111943

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NODAL (198-347) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NODAL
Gene ID: 4838
Uniprot ID: NODAL_HUMAN
Immunogen Region: 198-347
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 198-347 of human NODAL (NP_060525.3).
Immunogen Sequence: SNLSQEQRQLGGSTLLWEAE SSWRAQEGQLSWEWGKRHRR HHLPDRSQLCRKVKFQVDFN LIGWGSWIIYPKQYNAYRCE GECPNPVGEEFHPTNHAYIQ SLLKRYQPHRVPSTCCAPVK TKPLSMLYVDNGRVLLDHHK DMIVEECGCL
Function Essential for mesoderm formation and axial patterning during embryonic development.
Protein Name Nodal Homolog
Database Links Reactome: R-HSA-1181150
Reactome: R-HSA-1433617
Cellular Localisation Secreted
Alternative Antibody Names Anti-Nodal Homolog antibody
Anti-NODAL antibody

Information sourced from Uniprot.org

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