| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NME8 |
| Gene ID: | 51314 |
| Uniprot ID: | TXND3_HUMAN |
| Immunogen Region: | 299-588 |
| Immunogen Sequence: | FMDAFFPDFKKMKSMKLEKT LALLRPNLFHERKDDVLRII KDEDFKILEQRQVVLSEKEA QALCKEYENEDYFNKLIENM TSGPSLALVLLRDNGLQYWK QLLGPRTVEEAIEYFPESLC AQFAMDSLPVNQLYGSDSLE TAEREIQHFFPLQSTLGLIK PHATSEQREQILKIVKEAGF DLTQVKKMFLTPEQIEKIYP KVTGKDFYKDLLEMLSVGPS MVMILTKWNAVAEWRRLMG |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 299-588 of human NME8 (NP_057700.3). |
| Tissue Specificity | Testis-specific. Expressed only in primary spermatocytes and round spermatids. |
| Function | Probably required during the final stages of sperm tail maturation in the testis and/or epididymis, where extensive disulfide bonding of fibrous sheath (FS) proteins occurs. In vitro, it has neither nucleoside diphosphate kinase (NDPK) activity nor reducing activity on disulfide bonds. Exhibits a 3'-5' exonuclease activity with a preference for single-stranded DNA, suggesting roles in DNA proofreading and repair. |
| Protein Name | Thioredoxin Domain-Containing Protein 33'-5' Exonuclease Nme8Nm23-H8Nme/Nm23 Family Member 8Spermatid-Specific Thioredoxin-2Sptrx-2 |
| Database Links | |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Thioredoxin Domain-Containing Protein 3 antibodyAnti-3'-5' Exonuclease Nme8 antibodyAnti-Nm23-H8 antibodyAnti-Nme/Nm23 Family Member 8 antibodyAnti-Spermatid-Specific Thioredoxin-2 antibodyAnti-Sptrx-2 antibodyAnti-NME8 antibodyAnti-SPTRX2 antibodyAnti-TXNDC3 antibody |
Information sourced from Uniprot.org
![Western blot analysis of various lysates using [KO Validated] Lamin B1 Rabbit pAb (STJ119228) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98100/410505/STJ119228_1__00390.1744896984.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] Lamin B1 Rabbit pAb (STJ119228) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
