Anti-NME1 antibody (C-Term) (STJ73079)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ73079-100
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General Information

Short DescriptionGoat polyclonal antibody anti-NME1 (C-Term) is suitable for use in ELISA and Western Blot research applications.
ApplicationsPep-ELISA/WB
HostGoat
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 20+18kDa bands observed in lysates of cell lines A549, HeLa and HepG2 (calculated MW of 19.7kDa according to NP_937818.1 and 17.1kDa according to NP_000260.1). Recommended conc
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolNME1
Gene ID4830
Uniprot IDNDKA_HUMAN
Accession NumberNP_937818.1; NP_000260.1
Immunogen RegionC-Term
Immunogen SequenceDYTSCAQNWIYE
SpecificityThis antibody is expected to recognize both reported isoforms (NP_937818.1; NP_000260.1).

Additional Info

Function Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
Protein Name Nucleoside Diphosphate Kinase A
Ndk A
Ndp Kinase A
Granzyme A-Activated Dnase
Gaad
Metastasis Inhibition Factor Nm23
Nm23-H1
Tumor Metastatic Process-Associated Protein
Database Links Reactome: R-HSA-499943
Reactome: R-HSA-9748787
Reactome: R-HSA-9755088
Cellular Localisation Cytoplasm
Nucleus
Cell-Cycle Dependent Nuclear Localization Which Can Be Induced By Interaction With Epstein-Barr Viral Proteins Or By Degradation Of The Set Complex By Gzma
Alternative Antibody Names Anti-Nucleoside Diphosphate Kinase A antibody
Anti-Ndk A antibody
Anti-Ndp Kinase A antibody
Anti-Granzyme A-Activated Dnase antibody
Anti-Gaad antibody
Anti-Metastasis Inhibition Factor Nm23 antibody
Anti-Nm23-H1 antibody
Anti-Tumor Metastatic Process-Associated Protein antibody
Anti-NME1 antibody
Anti-NDPKA antibody
Anti-NM23 antibody

Information sourced from Uniprot.org

Citations

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