| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-NME1 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-0.01-0.03µg/mlELISA-antibody detection limit dilution 1:128000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
NME1 |
| Gene ID: |
4830 |
| Uniprot ID: |
NDKA_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_937818.1; NP_000260.1 |
| Specificity: |
This antibody is expected to recognize both reported isoforms (NP_937818.1; NP_000260.1). |
| Immunogen Sequence: |
DYTSCAQNWIYE |
| Function | Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. |
| Protein Name | Nucleoside Diphosphate Kinase ANdk ANdp Kinase AGranzyme A-Activated DnaseGaadMetastasis Inhibition Factor Nm23Nm23-H1Tumor Metastatic Process-Associated Protein |
| Database Links | Reactome: R-HSA-499943Reactome: R-HSA-9748787Reactome: R-HSA-9755088 |
| Cellular Localisation | CytoplasmNucleusCell-Cycle Dependent Nuclear Localization Which Can Be Induced By Interaction With Epstein-Barr Viral Proteins Or By Degradation Of The Set Complex By Gzma |
| Alternative Antibody Names | Anti-Nucleoside Diphosphate Kinase A antibodyAnti-Ndk A antibodyAnti-Ndp Kinase A antibodyAnti-Granzyme A-Activated Dnase antibodyAnti-Gaad antibodyAnti-Metastasis Inhibition Factor Nm23 antibodyAnti-Nm23-H1 antibodyAnti-Tumor Metastatic Process-Associated Protein antibodyAnti-NME1 antibodyAnti-NDPKA antibodyAnti-NM23 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and NME1/NM23A knockout (KO) 293T cells, using [KO Validated] NME1/NM23A Rabbit polyclonal antibody (STJ119972) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98757/382809/STJ119972_1__49721.1713151303.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NME1/NM23A knockout (KO) 293T cells, using [KO Validated] NME1/NM23A Rabbit polyclonal antibody (STJ119972) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")