| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:100-1:500 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NLRC4 |
| Gene ID: | 58484 |
| Uniprot ID: | NLRC4_HUMAN |
| Immunogen Region: | 85-170 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 85-170 of human NLRC4 (NP_001186067.1). |
| Immunogen Sequence: | NGQSLFHQTSEGDLDDLAQD LKDLYHTPSFLNFYPLGEDI DIIFNLKSTFTEPVLWRKDQ HHHRVEQLTLNGLLQALQSP CIIEGE |
| Tissue Specificity | Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain. |
| Post Translational Modifications | Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro. |
| Function | Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. |
| Protein Name | Nlr Family Card Domain-Containing Protein 4 Card - Lrr - And Nacht-Containing Protein Ced-4-Like Protein Clan Caspase Recruitment Domain-Containing Protein 12 Ice Protease-Activating Factor Ipaf |
| Database Links | Reactome: R-HSA-6803207 Reactome: R-HSA-844623 |
| Cellular Localisation | Cytoplasm Cytosol Inflammasome |
| Alternative Antibody Names | Anti-Nlr Family Card Domain-Containing Protein 4 antibody Anti-Card - Lrr - And Nacht-Containing Protein antibody Anti-Ced-4-Like Protein Clan antibody Anti-Caspase Recruitment Domain-Containing Protein 12 antibody Anti-Ice Protease-Activating Factor antibody Anti-Ipaf antibody Anti-NLRC4 antibody Anti-CARD12 antibody Anti-CLAN antibody Anti-CLAN1 antibody Anti-IPAF antibody Anti-UNQ6189 antibody Anti-PRO20215 antibody |
Information sourced from Uniprot.org