• Immunohistochemistry analysis of paraffin-embedded mouse pancreas using NKX6-1 antibody (STJ11103302) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NKX6-1 antibody (STJ11103302) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NKX6-1 antibody (1-100) (STJ11103302)

SKU:
STJ11103302

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NKX6-1
Gene ID: 4825
Uniprot ID: NKX61_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NKX6-1 (NP_006159.2).
Immunogen Sequence: MLAVGAMEGTRQSAFLLSSP PLAALHSMAEMKTPLYPAAY PPLPAGPPSSSSSSSSSSSP SPPLGTHNPGGLKPPATGGL SSLGSPPQQLSAATPHGIND
Tissue Specificity Pancreatic beta cells.
Function Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals.
Protein Name Homeobox Protein Nkx-6.1
Homeobox Protein Nk-6 Homolog A
Database Links Reactome: R-HSA-210745
Reactome: R-HSA-210747
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Nkx-6.1 antibody
Anti-Homeobox Protein Nk-6 Homolog A antibody
Anti-NKX6-1 antibody
Anti-NKX6A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance