| Short Description : | Rabbit polyclonal NKRF (391-690) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NKRF |
| Gene ID: | 55922 |
| Uniprot ID: | NKRF_HUMAN |
| Immunogen Region: | 391-690 |
| Immunogen Sequence: | YGTKKTSKHAAADEALKILQ KTQPTYPSVKSSQCHTGSSP RGSGKKKDIKDLVVYENSSN PVCTLNDTAQFNRMTVEYVY ERMTGLRWKCKVILESEVIA EAVGVKKTVKYEAAGEAVKT LKKTQPTVINNLKKGAVEDV ISRNEIQGRSAEEAYKQQIK EDNIGNQLLRKMGWTGGGLG KSGEGIREPISVKEQHKREG LGLDVERVNKIAKRDIEQII RNYARSESHTDLTFSRELT |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 391-690 of human NKRF (NP_060014.2). |
| Tissue Specificity | Widely and constitutively expressed. Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney. |
| Function | Enhances the ATPase activity of DHX15 by acting like a brace that tethers mobile sections of DHX15 together, stabilizing a functional conformation with high RNA affinity of DHX15. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription. May also act as a DNA-binding transcription regulator: interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. |
| Protein Name | Nf-Kappa-B-Repressing FactorNfkb-Repressing FactorNrfProtein Itba4 |
| Database Links | |
| Cellular Localisation | NucleusNucleolus |
| Alternative Antibody Names | Anti-Nf-Kappa-B-Repressing Factor antibodyAnti-Nfkb-Repressing Factor antibodyAnti-Nrf antibodyAnti-Protein Itba4 antibodyAnti-NKRF antibodyAnti-ITBA4 antibodyAnti-NRF antibody |
Information sourced from Uniprot.org
