• Western blot analysis of extracts of various cell lines, using NIF3L1 antibody (STJ118324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NIF3L1 antibody (1-377) (STJ118324)

SKU:
STJ118324

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NIF3L1 (1-377) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NIF3L1
Gene ID: 60491
Uniprot ID: NIF3L_HUMAN
Immunogen Region: 1-377
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human NIF3L1 (NP_001129511.1).
Immunogen Sequence: MLSSCVRPVPTTVRFVDSLI CNSSRSFMDLKALLSSLNDF ASLSFAESWDNVGLLVEPSP PHTVNTLFLTNDLTEEVMEE VLQKKADLILSYHPPIFRPM KRITWNTWKERLVIRALENR VGIYSPHTAYDAAPQGVNNW LAKGLGACTSRPIHPSKAPN YPTEGNHRVEFNVNYTQDLD KVMSAVKGIDGVSVTSFSAR TGNEEQTRINLNCTQKALMQ VVDFLSRNKQLYQKTEILS
Function May function as a transcriptional corepressor through its interaction with COPS2, negatively regulating the expression of genes involved in neuronal differentiation.
Protein Name Nif3-Like Protein 1
Amyotrophic Lateral Sclerosis 2 Chromosomal Region Candidate Gene 1 Protein
Cellular Localisation Cytoplasm
Nucleus
Interaction With Cops2 May Regulate Localization To The Nucleus
Alternative Antibody Names Anti-Nif3-Like Protein 1 antibody
Anti-Amyotrophic Lateral Sclerosis 2 Chromosomal Region Candidate Gene 1 Protein antibody
Anti-NIF3L1 antibody
Anti-ALS2CR1 antibody
Anti-MDS015 antibody
Anti-My018 antibody

Information sourced from Uniprot.org

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